Transgressive segregation reveals two Arabidopsis TIR-NB-LRR resistance genes effective against Leptosphaeria maculans, causal agent of blackleg disease

The Plant Journal : for Cell and Molecular Biology
Jens StaalChristina Dixelius

Abstract

In a cross between the two resistant accessions Col-0 and Ler-0, a 15:1 segregation was found in F2, suggesting the presence of unlinked resistance loci to Leptosphaeria maculans. One hundred Col-4 x Ler-0, and 50 Ler-2 x Cvi-1 recombinant inbred lines, and seven susceptible Ler-0 x Ws-0 F2 progenies were examined to identify the two loci. Resistance in Col-4, Ws-0 and Cvi-1 (RLM1) was mapped to the marker m305 on chromosome 1. Col-4 x Ler-0 and Ler-2 x Cvi-1 mapping populations located RLM2(Ler) on the same arm of chromosome 4. A tight physical location of RLM2 was established through near-isogenic lines. This region was found to correspond to an ancient duplication event between the RLM1 and RLM2 loci. Two independent T-DNA mutants in a TIR-NB-LRR R gene (At1g64070) displayed susceptibility, and L. maculans susceptible mutant phenotypes were confirmed to be allelic for rlm1 in F1 after crosses with susceptible rlm1(Ler)rlm2(Col) plants. Complementation of rlm1(Ler)rlm2(Col) with the genomic Col-0 sequence of At1g64070 conferred resistance. In addition, two T-DNA mutants in a neighbouring homologous TIR-NB-LRR gene (At1g63880) displayed moderate susceptibility to L. maculans. Sequence analysis revealed that At1g64070 was trunc...Continue Reading

References

May 1, 1990·The Plant Cell·E Koch, A Slusarenko
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·J Glazebrook, F M Ausubel
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·N AartsJ E Parker
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·B P ThommaW F Broekaert
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·J Vogel, S Somerville
Jun 16, 2001·Fungal Genetics and Biology : FG & B·B J HowlettM S Pedras
Nov 15, 2001·Mammalian Genome : Official Journal of the International Mammalian Genome Society·K F ManlyJ M Meer
Dec 26, 2001·Molecular Biology and Evolution·Erik RichlyDario Leister
Feb 16, 2002·Science·Cristina AzevedoPaul Schulze-Lefert
Feb 16, 2002·Science·Mark J AustinJane E Parker
Jun 18, 2002·Plant Physiology·Georg JanderRobert L Last
Sep 6, 2002·Genome Research·Mariana Mondragón-PalominoBrandon S Gaut
Sep 10, 2002·Trends in Biochemical Sciences·Sophie Janssens, Rudi Beyaert
Oct 9, 2002·The Plant Journal : for Cell and Molecular Biology·Blake C MeyersRichard W Michelmore
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S. BohmanC. Dixelius
Apr 3, 2003·The Plant Cell·Blake C MeyersRichard W Michelmore
May 7, 2003·Current Opinion in Biotechnology·Kim E Hammond-Kosack, Jane E Parker
May 27, 2003·Nature Reviews. Immunology·Naohiro Inohara, Gabriel Nuñez
Jun 5, 2003·The Plant Cell·William M GrayJane E Parker
Jun 14, 2003·Plant Physiology·Justin O Borevitz, Magnus Nordborg
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Akira TakahashiKen Shirasu
Oct 31, 2003·Nature·Nicholas C CollinsPaul Schulze-Lefert
Nov 18, 2003·Annual Review of Genetics·Zachary NimchukJeffery L Dangl
Apr 17, 2004·The Plant Journal : for Cell and Molecular Biology·Katherine J DenbyDaniel J Kliebenstein
May 29, 2004·The Plant Journal : for Cell and Molecular Biology·Eva SinapidouJim Beynon
Jun 23, 2004·The Plant Cell·Guillaume Blanc, Kenneth H Wolfe
Jul 10, 2004·Molecular Plant-microbe Interactions : MPMI·Mohammad H BorhanS Roger Rimmer
Sep 21, 2004·Annual Review of Plant Biology·Maarten KoornneefDick Vreugdenhil
Oct 27, 2004·The Plant Journal : for Cell and Molecular Biology·Paola VeroneseTesfaye Mengiste
Nov 2, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yo-Hei KanekoChikara Masuta

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Citations

May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Adnane NemriJonathan D G Jones
Apr 13, 2007·Molecular Plant-microbe Interactions : MPMI·Maria KaliffChristina Dixelius
May 21, 2010·PLoS Genetics·Daniela VladOlivier Loudet
Feb 3, 2009·PloS One·Sureshkumar BalasubramanianDetlef Weigel
Nov 19, 2008·Annual Review of Plant Biology·Ken Shirasu
Nov 5, 2014·BMC Plant Biology·Hanneke M PeeleChristina Dixelius
Jul 17, 2007·Current Opinion in Plant Biology·Eric B Holub
Jul 24, 2007·Trends in Plant Science·Jens Staal, Christina Dixelius
Sep 23, 2009·The Plant Journal : for Cell and Molecular Biology·Caroline LoutreBeat Keller
Jun 11, 2009·Communicative & Integrative Biology·Jens Staal, Christina Dixelius
Jan 3, 2012·Trends in Plant Science·Ishita AhujaAtle M Bones
Dec 19, 2014·Frontiers in Plant Science·Sophie J M PiquerezVardis Ntoukakis
May 24, 2016·Seminars in Cell & Developmental Biology·Elizabeth FrenchAnjali S Iyer-Pascuzzi
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Jun 6, 2018·International Journal of Molecular Sciences·Grazia M BorrelliAnna M Mastrangelo
Apr 10, 2008·The Plant Journal : for Cell and Molecular Biology·Jens StaalChristina Dixelius
Nov 24, 2007·FEMS Microbiology Letters·Robert P RyanDavid N Dowling
Jan 14, 2020·Plant, Cell & Environment·Prasanna KankanalaKirankumar S Mysore
Sep 3, 2019·Frontiers in Immunology·Annelies DemeyerRudi Beyaert
Sep 24, 2020·Molecular Biology and Evolution·Ana Cristina BarraganEunyoung Chae
Jan 6, 2021·International Journal of Molecular Sciences·Aldrin Y CantilaJacqueline Batley
Mar 27, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hua YangJacqueline Batley
Jul 10, 2021·Frontiers in Genetics·Papias H BinagwaConrad Bonsi

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