Transient Accumulation of Glycine Betaine and Dynamics of Endogenous Osmolytes in Salt-Stressed Cultures of Sinorhizobium meliloti.

Applied and Environmental Microbiology
R TalibartJ A Pocard

Abstract

The fate of exogenously supplied glycine betaine and the dynamics of endogenous osmolytes were investigated throughout the growth cycle of salt-stressed cultures of strains of Sinorhizobium meliloti which differ in their ability to use glycine betaine as a growth substrate, but not as an osmoprotectant. We present (sup13)C nuclear magnetic resonance spectral and radiotracer evidence which demonstrates that glycine betaine is only transiently accumulated as a cytoplasmic osmolyte in young cultures of wild-type strains 102F34 and RCR2011. Specifically, these strains accumulate glycine betaine as a preferred osmolyte which virtually prevents the accumulation of endogenous osmolytes during the lag and early exponential phases of growth. Then, betaine levels in stressed cells decrease abruptly during the second half of the exponential phase. At this stage, the levels of glutamate and the dipeptide N-acetylglutaminylglutamine amide increase sharply so that the two endogenous solutes supplant glycine betaine in the ageing culture, in which it becomes a minor osmolyte because it is progressively catabolized. Ultimately, glycine betaine disappears when stressed cells reach the stationary phase. At this stage, wild-type strains of S. mel...Continue Reading

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Citations

Jun 1, 2000·Applied and Environmental Microbiology·K GouffiC Blanco
Oct 20, 2009·Journal of Bacteriology·Ana Domínguez-FerrerasJuan Sanjuán
Jun 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Brice SagotGeneviève Alloing
Dec 29, 2010·Current Microbiology·Vida CasaitėRolandas Meškys
Jan 5, 2013·Current Microbiology·Sylwia Wdowiak-WróbelWanda Małek
Apr 3, 2007·Applied and Environmental Microbiology·Jan A C VriezenK Nüsslein
Jan 29, 2013·Applied and Environmental Microbiology·Matthew J Wargo
Feb 15, 2007·Environmental Microbiology·Mohammed EssendoubiMohamed Jebbar
Aug 3, 2010·FEBS Letters·Arnaud BriqueIsabelle Gosselin
Jan 1, 2010·Microbes and Environments·Waraporn TanthanuchNeung Teaumroong
Apr 24, 2007·Applied and Environmental Microbiology·Helen J McIntyreClive W Ronson
Dec 18, 2008·Applied and Environmental Microbiology·Arati V Patankar, Juan E González
Apr 2, 1999·Applied and Environmental Microbiology·K GouffiC Blanco
Sep 28, 1998·Journal of Bacteriology·K GouffiC Blanco
Aug 25, 2000·Journal of Basic Microbiology·M S DardanelliN E Ghittoni

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