Transient activation of fission yeast AMPK is required for cell proliferation during osmotic stress

Molecular Biology of the Cell
Katherine L Schutt, James B Moseley

Abstract

The heterotrimeric kinase AMPK acts as an energy sensor to coordinate cell metabolism with environmental status in species from yeast through humans. Low intracellular ATP leads to AMPK activation through phosphorylation of the activation loop within the catalytic subunit. Other environmental stresses also activate AMPK, but it is unclear whether cellular energy status affects AMPK activation under these conditions. Fission yeast AMPK catalytic subunit Ssp2 is phosphorylated at Thr-189 by the upstream kinase Ssp1 in low-glucose conditions, similar to other systems. Here we find that hyperosmotic stress induces strong phosphorylation of Ssp2-T189 by Ssp1. Ssp2-pT189 during osmotic stress is transient and leads to transient regulation of AMPK targets, unlike sustained activation by low glucose. Cells lacking this activation mechanism fail to proliferate after hyperosmotic stress. Activation during osmotic stress requires energy sensing by AMPK heterotrimer, and osmotic stress leads to decreased intracellular ATP levels. We observed mitochondrial fission during osmotic stress, but blocking fission did not affect AMPK activation. Stress-activated kinases Sty1 and Pmk1 did not promote AMPK activation but contributed to subsequent in...Continue Reading

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Citations

Feb 2, 2020·Applied and Environmental Microbiology·Rui YaoJing Wu
Apr 19, 2020·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Ying WangYingmei Wei
Jan 22, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Carlos SaldañaTeresa García-Gasca
Nov 11, 2019·Cell Systems·Benjamin D KnappFred Chang
Jul 21, 2021·ELife·Joseph O Magliozzi, James B Moseley

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Methods Mentioned

BETA
environmental stresses
salt stress
GTPases
PCR
Assay
protein assay

Software Mentioned

Insight
Image Studio Lite
StatPlus : Mac Analyst Soft
SoftWoRx
StatPlus Mac Analyst Soft
ImageJ

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