PMID: 4895538Jun 1, 1969Paper

Transient activation of RNA polymerase in Escherichia coli B after infection with bacteriophage T4

Proceedings of the National Academy of Sciences of the United States of America
A E OlesonJ M Buchanan

Abstract

Sköld and Buchanan(1) have reported that there is a rapid loss of RNA polymerase activity in Escherichia coli B after infection with T4 bacteriophage. More recent studies on the mechanism of this inactivation have been made in this(2) and other laboratories.(3, 4) In this communication, we report the observation of a transient stimulation of RNA polymerase activity when measurement is made immediately after infection and when cells are ruptured by a gentle lysis procedure. The increase in activity is independent of the synthesis of protein. The activity in the extracts of infected cells is lost by treatment of the extract with antibody to E. coli RNA polymerase and is refractive to the inhibitory action of the antibiotic rifamycin. Hybridization experiments indicate that an RNA transcribed almost exclusively from a T4 DNA template is the product of incubation of extracts of infected cells with a reaction mixture containing an exogenous primer (salmon sperm DNA). These findings are consistent with the hypothesis that one of the first steps in phage infection is the formation of a transcription complex containing T4 DNA and E. coli RNA polymerase.

References

Oct 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·A G SoA Tissières
Mar 18, 1968·Biochimica Et Biophysica Acta·A Sippel, G Hartmann
Feb 15, 1968·Biochemical and Biophysical Research Communications·G WalterW Zillig
Jul 26, 1968·Biochemical and Biophysical Research Communications·W WehrliM Staehelin
Jul 1, 1965·Journal of Molecular Biology·D Gillespie, S Spiegelman
Jun 28, 1968·Journal of Molecular Biology·K OkamotoJ Marmur
Dec 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·W MunyonJ T Grace
Sep 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·D D AnthonyD A Goldthwait
Feb 1, 1966·Analytical Biochemistry·S B Zimmerman, D Sandeen
Mar 21, 1967·Biochemical and Biophysical Research Communications·C Babinet
Feb 1, 1962·Proceedings of the National Academy of Sciences of the United States of America·J S WIBERGJ M BUCHANAN
May 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·H BREMER, M W KONRAD

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Citations

Aug 25, 1977·Journal of Molecular Biology·R J Frederick, L Snyder
Jun 9, 1972·Biochemical and Biophysical Research Communications·E HölttäJ Pispa
Jan 1, 1970·Archiv für die gesamte Virusforschung·R EnglerB Moore

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