Transient and stable transfection in the protozoan parasite Entamoeba invadens.

Molecular and Biochemical Parasitology
Gretchen M Ehrenkaufer, Upinder Singh

Abstract

Entamoeba histolytica is an important human pathogen and a major health problem worldwide. Many aspects of parasite biology can be studied with the exception of stage conversion, which cannot be reproduced adequately in E. histolytica. The reptile parasite Entamoeba invadens is a vital model system for studying stage conversion since it can be induced to undergo both encystation and excystation with high efficiency in vitro. However, functional studies using E. invadens have been limited by the lack of genetic tools in this species. Here, we report a new method for both transient and stable transfection of E. invadens. These new tools will greatly enhance research into Entamoeba development.

References

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Feb 11, 2012·Molecular and Biochemical Parasitology·Nishant SinghSudha Bhattacharya
Mar 20, 2012·Molecular and Biochemical Parasitology·Nishant SinghSudha Bhattacharya

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Citations

Jul 31, 2013·Genome Biology·Gretchen M EhrenkauferUpinder Singh
Jun 6, 2012·Current Opinion in Microbiology·Laura Morf, Upinder Singh
Jan 21, 2016·Infection and Immunity·Susmitha SureshUpinder Singh
Feb 9, 2021·PLoS Neglected Tropical Diseases·Conall SauveyRuben Abagyan
Dec 3, 2020·International Journal for Parasitology·Monica Mendes Kangussu-MarcolinoUpinder Singh

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