Transient corneal edema induced by nitric oxide synthase inhibition

Nitric Oxide : Biology and Chemistry
N YanagiyaJ Iwamoto

Abstract

The aim of the study is to identify nitric oxide synthase (NOS) in the rabbit cornea and further investigate the physiological role of nitric oxide in the rabbit cornea. For histological identification, an immunohistochemical technique using anti-NOS monoclonal antibodies was employed. For the physiological study, we measured the corneal thickness in vivo as an indicator of corneal edema by ultrasonic pachymetry. The measurements were repeated before and after ipsilateral injections of N(G)-nitro-L-arginine methyl ester (L-NAME) or N(G)-nitro-D-arginine methyl ester (D-NAME) or 6-anilino-5,8-quinolinedione (LY-83583) with contralateral injection of vehicle (balanced salt solution) into the anterior chamber of the rabbit. We also monitored intraocular pressure (IOP) by pneumatonometry. Endothelial NOS (eNOS) immunoreactivity was demonstrated both in the corneal epithelium and the endothelium. The corneal thickness significantly increased after L-NAME or LY-83583 without significant rise of IOP, whereas no change was detected after vehicle or D-NAME. These results suggest that NO is spontaneously produced in the corneal endothelium and the NO/cyclic GMP pathway is involved in maintainance of corneal thickness.

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Citations

Jul 28, 1999·European Journal of Pharmacology·M C Koss
Nov 26, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Junghyun KimJin Sook Kim
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Jan 23, 2013·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Isabella M Y CheungTrevor Sherwin
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May 4, 2021·The Ocular Surface·Shyam Sunder TummanapalliMark D P Willcox
Jul 3, 2021·Vision·Nir ErdinestNadav Levinger

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