Transient exposure of rhesus macaque oocytes to calyculin-A and okadaic acid stimulates germinal vesicle breakdown permitting subsequent development and fertilization

Biology of Reproduction
G D SmithD P Wolf

Abstract

Exposure of mammalian oocytes to the protein phosphatase (PP)-1 (PP1) and PP2A inhibitor okadaic acid (OA) stimulates oocyte meiosis. However, treated oocytes do not develop beyond metaphase I (MI), and they display morphological aberrations. Experiments were conducted to define inhibitor treatment conditions for macaque oocytes that would result in germinal vesicle breakdown (GVB) stimulation and completion of meiosis without significant cytoplasmic abnormalities. As described above for OA, continual exposure of macaque oocytes to 50 nM calyculin-a (CL-A) significantly enhanced GVB at 24 h compared to that in controls, and the majority of the treated oocytes displayed cytoplasmic abnormalities. However, transient exposure (10 min) of rhesus macaque oocytes to either 50 nM CL-A or 1.0 microM OA enhanced GVB rates compared to that in controls and did not increase the incidence of cytoplasmic abnormalities. Meiotic maturation from germinal vesicle-intact oocytes to MII was enhanced following transient treatment with CL-A or OA compared to that in controls; however, development from MI to MII occurred at a similar frequency. In vitro-matured oocytes transiently exposed to OA and CL-A were capable of fertilization. In addition, ova...Continue Reading

Citations

Jan 26, 2010·Marine Drugs·Francesco Silvestre, Elisabetta Tosti
May 19, 2010·Marine Drugs·Francesco Silvestre, Elisabetta Tosti
Oct 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Xia WangGary D Smith
Jan 12, 1999·Developmental Biology·G D SmithD P Wolf
Mar 27, 2003·Molecular Reproduction and Development·Jason E SwainGary D Smith
Dec 1, 2004·Molecular Reproduction and Development·Cean LaRosa, Stephen M Downs

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