Transient expression analysis of the mouse ornithine decarboxylase antizyme haploid-specific promoter using in vivo electroporation

FEBS Letters
Akiko IkeMasami Nozaki


The testicular isoform of the ornithine decarboxylase antizyme (OAZt) gene is expressed exclusively in the haploid spermatids of mice. The 357-bp region, which includes a TATA-less promoter and an untranslated region, is sufficient for OAZt gene expression in the spermatids of transgenic mice. In this study, in vivo transient transfection to living mouse testes was used to define the transcriptional regulatory elements of the OAZt gene promoter. We found that the 10-bp element that contains an initiator (Inr) plays a central role as the core promoter, in combination with a downstream element, while two cyclic adenosine monophosphate-responsive element (CRE)-like sites in the upstream region also contribute to promoter activity. The electrophoretic mobility shift assay showed binding of the testis-specific factors to these elements. Our results show that the in vivo DNA transfer technique enables detailed analysis of haploid germ cell-specific gene regulation in mice.


Oct 18, 2011·Asian Journal of Andrology·Zhen ZhangFei Zhou
Dec 7, 2007·Biology of Reproduction·HuangHui TangErwin Goldberg
May 17, 2008·Development, Growth & Differentiation·Kentaro Yomgogida
Jul 16, 2008·Reproductive Biology and Endocrinology : RB&E·Emilio González-GonzálezJesús Del Mazo
Jul 1, 2009·Reproductive Biology and Endocrinology : RB&E·Sandra DannerRichard Ivell
Sep 14, 2007·Archives of Andrology·Kevin CowardJohn Parrington
Jan 13, 2011·Systems Biology in Reproductive Medicine·John ParringtonJoaquin Gadea
Jun 14, 2005·Developmental Biology·Masanobu ShojiNorio Nakatsuji
Feb 7, 2006·The Journal of Biological Chemistry·Anthony E Pegg


Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J J PeschonR D Palmiter
Dec 22, 1995·The Journal of Biological Chemistry·T A Ince, K W Scotto
Mar 14, 1996·Nature·F NantelPaolo Sassone-Corsi
Feb 1, 1997·Molecular and Cellular Biology·R C IannelloI Kola
Sep 2, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N B Hecht
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·B A LewisS H Orkin
Jun 10, 2000·Molecular and Cellular Biology·A K Kutach, James T Kadonaga
Apr 17, 2002·Endocrinology·Ans M M van PeltFederica M F van Dissel-Emiliani
Mar 26, 2003·Annual Review of Biochemistry·Stephen T Smale, James T Kadonaga
Apr 3, 2003·Microscopy Research and Technique·Jean-Pierre Dadoune

Related Concepts

Benign Neoplasm of Testis
Untranslated Regions
Physiologic Pulse
Nuclear Proteins
CREM gene
ODC-p protein, human
Neoplasm of Uncertain or Unknown Behavior of Testis
Complex (molecular entity)

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