Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector

Virus Research
Arvind VarsaniEdward P Rybicki

Abstract

A Tobacco mosaic virus (TMV)-derived vector was used to express a native Human papillomavirus type 16 (HPV-16) L1 gene in Nicotiana benthamiana by means of infectious in vitro RNA transcripts inoculated onto N. benthamiana plants. HPV-16 L1 protein expression was quantitated by enzyme-linked immunosorbent assays (ELISA) after concentration of the plant extract. We estimated that the L1 product yield was 20-37 microg/kg of fresh leaf material. The L1 protein in the concentrated extract was antigenically characterised using the neutralising and conformation-specific Mabs H16:V5 and H16:E70, which bound to the plant-produced protein. Particles observed by transmission electron microscopy were mainly capsomers but virus-like particles (VLPs) similar to those produced in other systems were also present. Immunisation of rabbits with the concentrated plant extract induced a weak immune response. This is the first report of the successful expression of an HPV L1 gene in plants using a plant virus vector.

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Citations

Sep 14, 2007·BMC Biotechnology·Thomas O KohlEdward P Rybicki
Jun 25, 2008·BMC Biotechnology·Ann MeyersEdward P Rybicki
Feb 25, 2014·Clinics in Dermatology·Lis Ribeiro-Müller, Martin Müller
Nov 6, 2008·Drug Discovery Today·Edward P Rybicki
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Nov 11, 2015·Human Vaccines & Immunotherapeutics·Edward Rybicki
Aug 30, 2006·Virus Research·Arvind VarsaniEdward P Rybicki
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Aug 3, 2010·Expert Review of Vaccines·Tatiana V KomarovaYuri L Dorokhov

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