Abstract
The aim of this study is to investigate the presence of orbital lymphatic vessels during fetal and neonatal development and in adults using a panel of lymphatic markers. This was a retrospective observational case series. For analyzing lymphatic vessels, we used formalin-fixed paraffin-embedded enucleated eyes from 25 human fetuses between 13 and 24 weeks of gestation and postnatal eyes from 15 children and 5 adults. Immunohistochemical analysis of lymphatic vessels was performed for the markers: lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1), podoplanin (D2-40), Prospero-related homeobox gene-1 (Prox-1), pan-endothelial marker CD31, and blood vessel endothelium specific CD34. Vasculature showing endothelial expression of LYVE-1, D2-40, Prox-1, and CD31 in combination with absence or weak expression of CD34, as would be expected for lymphatic vessels, was seen in 11 of 25 fetuses in an age range from 14 weeks to 23 weeks of gestation (44%). This lymphatic vascular staining pattern was also observed in 4 of 15 liveborn children (27%), all within 1 month of age, of which two were born prematurely at 32 and 34 weeks of gestation. Interestingly, an incomplete lymphatic staining pattern was observed in another 4 fe...Continue Reading
References
Oct 1, 1993·The American Journal of Surgical Pathology·S W Weiss, B J Nickoloff
Jul 11, 2000·Nature Genetics·B Sosa-PinedaG Oliver
Nov 2, 2001·The American Journal of Surgical Pathology·J K McKenneyA L Folpe
Jan 16, 2002·Anatomy and Embryology·M Rodriguez-NiedenführJ Wilting
Aug 20, 2004·Biology of Reproduction·Sharon Gerecht-NirJoseph Itskovitz-Eldor
Oct 20, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marc P PusztaszeriFred T Bosman
Mar 2, 2006·The American Journal of Pathology·Ulrike FiedlerHellmut G Augustin
Oct 5, 2006·Eye·A J Dickinson, R E Gausas
Nov 2, 2006·Cornea·Claus CursiefenFalk Schroedl
Nov 23, 2006·British Journal of Cancer·I Van der AuweraP B Vermeulen
May 16, 2008·American Journal of Clinical Pathology·Chris H JokinenBrian P Rubin
Aug 12, 2008·Investigative Ophthalmology & Visual Science·Falk SchroedlClaus Cursiefen
Sep 4, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hanspeter E KillerPeter Meyer
Aug 27, 2010·Investigative Ophthalmology & Visual Science·Hui ZhangLu Chen
Nov 1, 2011·Journal of Anatomy·Kwang Ho ChoYukio Katori
Jan 4, 2012·Journal of Thrombosis and Haemostasis : JTH·A M KerriganG D Brown
Apr 24, 2012·Journal of Ophthalmology·Shintaro NakaoTatsuro Ishibashi
Aug 14, 2012·Advances in Anatomic Pathology·Nelson G Ordóñez
Jul 1, 2014·Experimental Eye Research·Simona L SchlerethLudwig M Heindl
Oct 16, 2014·Investigative Ophthalmology & Visual Science·Falk SchroedlClaus Cursiefen
Dec 27, 2015·Survey of Ophthalmology·Jessica F YangJin-Hong Chang
May 14, 2016·Virchows Archiv : an International Journal of Pathology·Lukasz A AdamczykUlrik Baandrup
Jul 18, 2016·Ophthalmology·Lindsay L WongLeo A Kim
Oct 11, 2016·Stem Cell Reports·Bin MaoFeng Ma
Apr 4, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Neil I Bower, Benjamin M Hogan
Feb 20, 2019·Cancers·Jackelien G M van BeekRobert M Verdijk