Transient Expression of Secretory IgA In Planta is Optimal Using a Multi-Gene Vector and may be Further Enhanced by Improving Joining Chain Incorporation

Frontiers in Plant Science
Lotte B WesterhofArjen Schots

Abstract

Secretory IgA (sIgA) is a crucial antibody in host defense at mucosal surfaces. It is a promising antibody isotype in a variety of therapeutic settings such as passive vaccination and treatment of inflammatory disorders. However, heterologous production of this heteromultimeric protein complex is still suboptimal. The challenge is the coordinate expression of the four required polypeptides; the alpha heavy chain, the light chain, the joining chain, and part of the polymeric-Ig-receptor called the secretory component, in a 4:4:1:1 ratio. We evaluated the transient expression of three sIgAκ variants, harboring the heavy chain isotype α1, α2m1, or α2m2, of the clinical antibody Ustekinumab in planta. Ustekinumab is directed against the p40 subunit that is shared by the pro-inflammatory cytokines interleukin (IL)-12 and IL-23. A sIgA variant of this antibody may enable localized treatment of inflammatory bowel disease. Of the three different sIgA variants we obtained the highest yield with sIgA1κ reaching up to 373 μg sIgA/mg total soluble protein. The use of a multi-cassette vector containing all four expression cassettes was most efficient. However, not the expression strategy, but the incorporation of the joining chain turned ou...Continue Reading

References

Oct 15, 1990·The Biochemical Journal·M A Kerr
Jun 1, 1987·Annals of Internal Medicine·M E Conley, D L Delacroix
Jul 1, 1969·The Journal of Allergy·S C MinesP Fireman
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·J BerdozB Corthésy
Oct 13, 2001·The New England Journal of Medicine·J KeaneM M Braun
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Olivier VoinnetDavid Baulcombe
Mar 15, 2003·The Journal of Biological Chemistry·Louise RoylePauline M Rudd
Feb 2, 2006·Journal of Biotechnology·Willemien H WielandDiego V Orzáez
Feb 6, 2010·Plant Biotechnology Journal·Benoit De MuynckMarc Boutry
Apr 2, 2010·Journal of the American Chemical Society·Yoshiki NarimatsuKazuhiko Yamasaki
Feb 16, 2012·The Journal of Biological Chemistry·Zhang YangBent L Petersen
Sep 6, 2012·The Journal of Biological Chemistry·Alexandra CastilhoRichard Strasser
Sep 22, 2012·Human Vaccines & Immunotherapeutics·Qiang Chen, Huafang Lai
Feb 12, 2013·Current Pharmaceutical Design·Dirk BoschHerta Steinkellner
Mar 20, 2013·International Journal of Molecular Sciences·Paloma JuarezDiego Orzaez
Aug 21, 2013·Inflammatory Bowel Diseases·Kailash C BholBarbara S Fox
Apr 23, 2014·Nature Reviews. Immunology·Markus F Neurath
Sep 10, 2014·Plant Biotechnology Journal·Lotte B WesterhofArjen Schots

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Protein Assay
glycosylation

Software Mentioned

pRAPa
GeneArt

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Molecular Sciences
Paloma JuarezDiego V Orzáez
Gastroenterología y hepatología
Cristina Pisabarros BlancoFernando Muñoz Nuñez
© 2022 Meta ULC. All rights reserved