Transient focal membrane deformation induced by arginine-rich peptides leads to their direct penetration into cells.

Molecular Therapy : the Journal of the American Society of Gene Therapy
Hisaaki HiroseShiroh Futaki

Abstract

Endocytosis has been implicated in the cellular uptake of arginine-rich, cell-penetrating peptides (CPPs). However, accumulating evidence suggests that certain conditions allow the direct, non-endocytic penetration of arginine-rich peptides through the plasma membrane. We previously showed that Alexa Fluor 488-labeled dodeca-arginine (R12-Alexa488) directly enters cells at specific sites on the plasma membrane and subsequently diffuses throughout cells. In this study, we found that the peptide influx was accompanied by the formation of unique, "particle-like" multivesicular structures on the plasma membrane, together with topical inversion of the plasma membrane. Importantly, the conjugation of dodeca-arginine (R12) to Alexa Fluor 488 or a peptide tag derived from hemagglutinin (HAtag) significantly accelerated particle formation, suggesting that the chemical properties of the attached molecules (cargo molecules) may contribute to translocation of the R12 peptide. Coincubation with R12-HAtag allowed the membrane-impermeable R4-Alexa488 to permeate cells. These results suggest that R12 peptides attached to hydrophobic cargo molecules stimulate dynamic morphological alterations in the plasma membrane, and that these structural ch...Continue Reading

References

Feb 25, 1997·Biochemistry·K MatsuzakiK Miyajima
Jun 5, 1997·Nature·K Simons, E Ikonen
Mar 28, 1998·The Journal of Biological Chemistry·A YamajiM Umeda
Oct 29, 2000·Archives of Biochemistry and Biophysics·C D'AntuonoD L Bernik
Jan 18, 2002·The Journal of Biological Chemistry·L Ruth MontesAlicia Alonso
Nov 2, 2002·The Journal of Biological Chemistry·Jean Philippe RichardBernard Lebleu
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Paul L McNeil, Richard A Steinhardt
Mar 25, 2004·Nature Cell Biology·Alain Joliot, Alain Prochiantz
Nov 27, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ikuhiko NakaseShiroh Futaki
Sep 21, 2006·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Sergii AfoninAnne S Ulrich
Oct 31, 2006·Biochimica Et Biophysica Acta·Katsumi MatsuzakiHironobu Naiki
Sep 1, 2007·Journal of Cellular and Molecular Medicine·Arwyn Tomos Jones
Oct 26, 2007·Journal of Cell Science·Stella TournavitiOliver T Fackler
Jan 1, 2008·Advanced Drug Delivery Reviews·Paul A WenderThomas H Pillow
Apr 10, 2008·PloS One·Antonin LamazièreJesus Ayala-Sanmartin
Sep 13, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Caroline Palm-ApergiMattias Hällbrink
Dec 2, 2008·Nature Cell Biology·Chuanxi CaiJianjie Ma
Feb 11, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sayaka KatayamaShiroh Futaki
Feb 17, 2010·The Journal of Cell Biology·Mirkka KoivusaloSergio Grinstein
Jul 14, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wouter P R VerdurmenRoland Brock
Feb 16, 2011·Journal of Cellular Biochemistry·Dong-Wook RyuMinhyung Lee

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Citations

Sep 7, 2013·Accounts of Chemical Research·Federica SgolastraGregory N Tew
Apr 5, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Caroline Palm-ApergiSteven F Dowdy
Feb 4, 2014·PloS One·Tomoyuki Tsumuraya, Masayuki Matsushita
Dec 11, 2014·Therapeutic Delivery·Janely Pae, Margus Pooga
May 15, 2013·FEBS Letters·Chérine Bechara, Sandrine Sagan
Jun 15, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ying ShenHarald Sauthoff
Aug 21, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Soo Hyeon LeeJean-Christophe Leroux
Nov 4, 2015·Basic & Clinical Pharmacology & Toxicology·Mie Kristensen, Hanne Mørck Nielsen
Sep 10, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jamie BrugnanoAlyssa Panitch
Mar 29, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Marco LelleKalina Peneva
Mar 24, 2016·Biochimica Et Biophysica Acta·Yuki Takechi-HarayaHiroyuki Saito
Sep 1, 2015·Acta histochemica·Ahmed A M Abdel-Hamid, Alaa El-Din L Firgany
Jul 27, 2015·Pharmacology & Therapeutics·Joshua D Ramsey, Nicholas H Flynn
May 28, 2013·Biochimica Et Biophysica Acta·Margarida RodriguesNuno C Santos
Mar 4, 2014·The International Journal of Biochemistry & Cell Biology·Ofelia ManitiJesus Ayala-Sanmartin
Apr 15, 2016·IET Nanobiotechnology·Samad Mussa FarkhaniHadi Valizadeh
May 23, 2015·Therapeutic Delivery·Jagadish BeloorPriti Kumar
Mar 12, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vijaya Gopal
Jun 19, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Afrouz YousefiEnrico Mastrobattista
May 15, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wouter P R VerdurmenRoland Brock
May 18, 2016·Analytica Chimica Acta·Kazuharu SugawaraHideki Kuramitz
Sep 7, 2016·Journal of Chemical Theory and Computation·Hui-Yuan ZhangDong-Qing Wei
Oct 12, 2016·Chemical & Pharmaceutical Bulletin·Toshihide Takeuchi, Shiroh Futaki
Mar 30, 2017·ACS Chemical Biology·Ting-Yi WangJean-Philippe Pellois
May 16, 2017·Gene Therapy·C ZylberbergS Matosevic
Jun 10, 2017·Angewandte Chemie·Tomo MurayamaShiroh Futaki
Dec 14, 2016·Bioorganic & Medicinal Chemistry Letters·Toshihiko Tashima

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