Transient genetic transformation of Mougeotia scalaris (Zygnematophyceae) mediated by the endogenous α-tubulin1 promoter

Journal of Phycology
Merle RegensdorffHenrik Buschmann

Abstract

Mougeotia scalaris is a filamentous streptophyte alga renowned for light-inducible plastid rotation and microtubule-dependent polarity establishment. As a first step toward transgenic approaches we determined the 5,825 base pair genomic sequence encoding the α-tubulin1 gene (MsTUA1) of M. scalaris (strain SAG 164.80). The subcloned MsTUA1 promoter facilitated strong transgene expression in M. scalaris and tobacco leaf cells, as shown by particle bombardment and the subsequent visualization of expressed fluorescent protein markers. Our results provide a route for the genetic transformation of the filamentous streptophyte alga M. scalaris based on the endogenous TUA1 promoter.

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Citations

Feb 27, 2020·Journal of Experimental Botany·Lenka Caisová
Feb 23, 2020·Journal of Experimental Botany·Hong Zhou, Klaus von Schwartzenberg
Apr 5, 2020·Journal of Experimental Botany·Stanislav VosolsoběJan Petrášek
Jun 13, 2020·Journal of Experimental Botany·Henrik Buschmann, Andreas Holzinger
Jul 28, 2021·Plant Reproduction·Stefan A Rensing, Dolf Weijers

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