Transient heat release during induced mitochondrial proton uncoupling

Communications Biology
Manjunath C RajagopalSanjiv Sinha

Abstract

Non-shivering thermogenesis through mitochondrial proton uncoupling is one of the dominant thermoregulatory mechanisms crucial for normal cellular functions. The metabolic pathway for intracellular temperature rise has widely been considered as steady-state substrate oxidation. Here, we show that a transient proton motive force (pmf) dissipation is more dominant than steady-state substrate oxidation in stimulated thermogenesis. Using transient intracellular thermometry during stimulated proton uncoupling in neurons of Aplysia californica, we observe temperature spikes of ~7.5 K that decay over two time scales: a rapid decay of ~4.8 K over ~1 s followed by a slower decay over ~17 s. The rapid decay correlates well in time with transient electrical heating from proton transport across the mitochondrial inner membrane. Beyond ~33 s, we do not observe any heating from intracellular sources, including substrate oxidation and pmf dissipation. Our measurements demonstrate the utility of transient thermometry in better understanding the thermochemistry of mitochondrial metabolism.

References

Jan 1, 1979·Methods in Enzymology·P G Heytler
Oct 18, 1991·Biochimica Et Biophysica Acta·B Korzeniewski, W Froncisz
Mar 1, 1986·The Journal of Cell Biology·T KreinerR H Scheller
Feb 1, 1973·Biological Reviews of the Cambridge Philosophical Society·L Janský
Jan 1, 1984·Physiological Reviews·D G Nicholls, R M Locke
Dec 30, 1981·Biochimica Et Biophysica Acta·H Terada
Jan 6, 1999·Biophysical Journal·R C Scaduto, L W Grotyohann
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·C JollyR I Morimoto
Jul 6, 2002·Critical Reviews in Oncology/hematology·Bert HildebrandtHanno Riess
Jan 15, 2003·Experimental Physiology·Martin Klingenspor
Jan 1, 1963·Zeitschrift für Zellforschung und mikroskopische Anatomie·J ROSENBLUTH
Oct 15, 2005·Nature Reviews. Neuroscience·Zane B AndrewsTamas L Horvath
Jan 15, 2010·Journal of Neurophysiology·Charlene M HickeyNeil S Magoski
Jun 23, 2010·The Quarterly Review of Biology·Mauro MandrioliDario Sonetti
Jan 25, 2011·Current Biology : CB·Leonid L Moroz
Mar 8, 2012·Nano Letters·Jon S DonnerRomain Quidant
Nov 8, 2012·PLoS Computational Biology·Valerii M SukhorukovMichael Meyer-Hermann
May 2, 2013·Cellular and Molecular Life Sciences : CMLS·Artem K VelichkoOmar L Kantidze
Mar 19, 2014·Molecular Metabolism·Brandon M KenwoodKyle L Hoehn
Jul 11, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel W SummersJeffrey Milbrandt
Aug 29, 2014·Nature Methods·Guillaume BaffouLudovic Jullien
Sep 1, 2015·Nature Methods·Shigeki KiyonakaYasuo Mori
Sep 1, 2015·Nature Methods·Madoka SuzukiShin'ichi Ishiwata
Mar 2, 2016·Scientific Reports·Ryuichi TanimotoAkira Funahashi
Nov 24, 2016·Small·Rami T ElAfandyBoon S Ooi
Sep 20, 2017·Molecular Neurodegeneration·Rebecca San GilHeath Ecroyd
Dec 15, 2017·Biophysical Reports·Tao-Rong XieJian-Sheng Kang
Dec 22, 2017·Signal Transduction and Targeted Therapy·Tao-Rong XieJian-Sheng Kang
May 15, 2018·Biophysical Reports·Jian-Sheng Kang
Nov 23, 2018·Nature Communications·Andrea D PickelChris Dames

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Citations

Mar 17, 2020·Biophysics Reviews·Madoka Suzuki, Taras Plakhotnik
Sep 30, 2020·Nature Methods·Jiajia ZhouDayong Jin
Jan 31, 2021·The Review of Scientific Instruments·Manjunath C Rajagopal, Sanjiv Sinha
Feb 8, 2020·Molecular Aspects of Medicine·L DardR Rossignol
Nov 17, 2020·ACS Sensors·Fangxu WangNing Gu

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