Transient Ion-Pair Separations for Electrospray Mass Spectrometry

Analytical Chemistry
Hanghui LiuPurnendu K Dasgupta

Abstract

We report a novel ion-pair chromatography (IPC) approach for liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS), where the eluent does not contain any ion-pairing reagent (IPR). The IPR is injected on the column, much like the sample, and moves down the column. Significant amounts of a high retention factor IPR is injected, resulting in a transient but reproducible regional coating that progresses along the column. The sample is injected after a brief interval. The sample components interact with the IPR coated region during their passage; the chosen eluent gradient elutes the analytes of interest into the mass spectrometer before the IPR. Following analyte elution, the gradient is steeply raised, the IPR is washed out, and the effluent is sent to waste via a diverter valve until it is fully removed. As the nature of the analyte retention continuously changes along the column and with time, we call this transient ion-pair separation (TIPS). As the IPR never enters the MS, TIPS addresses two major drawbacks of IPC for ESI-MS: it avoids both ion suppression and ion source contamination. The potential of the generic approach for other modes of separation is discussed. An illustrative separation of two smal...Continue Reading

References

Mar 1, 1997·The American Journal of Hospice & Palliative Care·J R Snyder
May 27, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wenyun LuJoshua D Rabinowitz

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Citations

May 4, 2020·Journal of Chromatography. a·Aurore DesmonsFathi Moussa
Mar 25, 2021·Analytical Chemistry·Charles Phillip ShelorPurnendu K Dasgupta

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