Transient knockdown-mediated deficiency in plectin alters hepatocellular motility in association with activated FAK and Rac1-GTPase

Cancer Cell International
Chiung-Chi ChengYi-Hsiang Liu

Abstract

Plectin is one of the cytolinker proteins that play a crucial role in maintaining the integrity of cellular architecture. It is a component of desmosome complexes connecting cytoskeletal proteins and trans-membrane molecules. In epithelial cells, plectin connects cytokeratins and integrin α6β4 in hemidesmosomes anchoring to the extracellular matrix. In addition to the function of molecular adherent, plectin has been reported to exhibit functions affecting cellular signals and responsive activities mediated by stress, cellular migration, polarization as well as the dynamic movement of actin filaments. Plectin deficiency in hepatocellular carcinoma results in abnormal expression of cytokeratin 18 and disassembled hemidesmosome. Therefore, it is hypothesized that the plectin deficiency-mediated collapse of cytoskeleton may modulate cellular motility that is associated with consequent metastatic behaviors of cancer cells. The cellular motility of plectin-deficient Chang liver cells generated by transient knockdown were analyzed by trans-well migration assay and the results revealed a higher migration rate. The confocal microscopy also demonstrated less organized and more polarized morphology as well as more focal adhesion kinase ac...Continue Reading

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Citations

Mar 29, 2018·International Journal of Molecular Sciences·Lifang HuAirong Qian
Jul 17, 2020·Disease Markers·Xiaojing YangJie Fu
Mar 10, 2017·Cell Adhesion & Migration·Chiung-Chi ChengYi-Hsiang Liu

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Methods Mentioned

BETA
pull-down
GTPase
surgical resection
Assay

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