Transient Middle Cerebral Artery Occlusion Model of Neonatal Stroke in P10 Rats

Journal of Visualized Experiments : JoVE
Amara Larpthaveesarp, Fernando F Gonzalez

Abstract

A number of animal models have been used to study hypoxic-ischemic injury, traumatic injury, global hypoxia, or permanent ischemia in both the immature and mature brain. Stroke occurs commonly in the perinatal period in humans, and transient ischemia-reperfusion is the most common form of stroke in neonates. The reperfusion phase is a critical component of injury progression, which occurs over a period of days to weeks, and of the endogenous response to injury. This postnatal day 10 (p10) rat model of transient middle cerebral artery occlusion (tMCAO) creates a unilateral, non-hemorrhagic focal ischemia-reperfusion injury that can be utilized to study the mechanisms of focal injury and repair in the full-term-equivalent brain. The injury pattern that is produced by tMCAO is consistent and highly reproducible and can be confirmed with MRI or histological analyses. The severity of injury can be manipulated through changes in occlusion time and other methods that will be discussed.

Citations

Sep 15, 2020·Experimental Neurobiology·Jinyoung WonYoungjeon Lee
May 17, 2019·Neural Plasticity·Mariangela GennaroTommaso Pizzorusso
Sep 25, 2019·International Journal of Molecular Sciences·Jenia Kouchek ZadehAdrian Gericke
Sep 6, 2020·Experimental Neurology·Nancy HamdyZhong-Ping Feng
Aug 4, 2021·Molecular and Cellular Neurosciences·Vien NguyenYerem Yeghiazarians

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