PMID: 7536022Apr 1, 1995Paper

Transient perfusion in human melanoma xenografts

British Journal of Cancer
I Tufto, E K Rofstad

Abstract

Studies of transplantable rodent tumours have suggested that malignant tissue might experience transient perfusion at the microvascular level. The purpose of the work reported here was to investigate whether transient perfusion can be demonstrated in xenografted human tumours. Tumours of four melanoma lines (A-07, D-12, R-18, U-25), grown orthotopically in Balb/c nu/nu mice, were included in the study. Transient perfusion was studied by using the double-fluorescent staining technique. Hoechst 33342 and DiOC7(3) were either administered simultaneously or Hoechst 33342 was administered 20 min before DiOC7(3). Detection of transient perfusion by this method requires that vessels are non-functional for at least 5 min owing to the distribution half-lives of the dyes in the blood. Usable combinations of dye concentrations were found by varying the concentrations of Hoechst 33342 and DiOC7(3) systematically. The level of perfusion mismatch following simultaneous administration of the dyes ranged from approximately 1.5% for U-25 tumours to approximately 3.0% for R-18 tumours at these combinations. Moreover, the fraction of vessels stained only with Hoechst 33342 and the fraction of vessels stained only with DiOC7(3) were not significan...Continue Reading

Citations

Mar 26, 2011·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Constantin-Alin MafteiPeter Vaupel
Sep 1, 2007·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Kjetil G BrurbergEinar K Rofstad
Mar 29, 2012·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·C Bayer, P Vaupel
Nov 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L A BroströmL Boquist
Sep 13, 2012·Experimental and Therapeutic Medicine·Takahiro OikeTakashi Nakano
Jul 18, 2008·Cancer Research·Laura I Cárdenas-NaviaMark W Dewhirst
Sep 3, 2004·Cancer Research·L Isabel Cárdenas-NaviaMark W Dewhirst

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