Transient receptor potential channel 1 maintains adherens junction plasticity by suppressing sphingosine kinase 1 expression to induce endothelial hyperpermeability

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Mohammad TauseefDolly Mehta

Abstract

Stability of endothelial cell (EC) adherens junctions (AJs) is central for prevention of tissue edema, the hallmark of chronic inflammatory diseases including acute respiratory distress syndrome. Here, we demonstrate a previously unsuspected role of sphingosine kinase 1 (SPHK1) in the mechanism by which transient receptor potential channel 1 (Trpc1)-mediated Ca(2+) entry destabilizes AJs. Trpc1(-/-) monolayers showed a 2.2-fold increase in vascular endothelial (VE)-cadherin cell-surface expression above wild-type (WT) monolayers. Thrombin increased endothelial permeability (evident by a 5-fold increase in interendothelial gap area and 60% decrease in transendothelial electrical resistance) in WT but not Trpc1(-/-) ECs. Trpc1(-/-) mice resisted the hyperpermeability effects of the edemagenic agonists used and exhibited 60% less endotoxin-induced mortality. Because sphingosine-1-phosphate (S1P) strengthens AJs, we determined if TRPC1 functioned by inhibiting SPHK1 activity, which generates S1P. Intriguingly, Trpc1(-/-) ECs or ECs transducing a TRPC1-inactive mutant showed a 1.5-fold increase in basal SPHK1 expression compared with WT ECs, resulting in a 2-fold higher S1P level. SPHK1 inhibitor SK1-I decreased basal transendotheli...Continue Reading

References

Oct 16, 2002·The Journal of Biological Chemistry·Monu GoelWilliam P Schilling
Mar 16, 2004·The Journal of Biological Chemistry·Gias U AhmmedAsrar B Malik
Jul 30, 2004·American Journal of Respiratory and Critical Care Medicine·Bryan J McVerryJoe G N Garcia
Sep 7, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Biman C PariaChinnaswamy Tiruppathi
Jun 23, 2005·Pflügers Archiv : European journal of physiology·Alexander DietrichThomas Gudermann
Aug 25, 2005·The Journal of Biological Chemistry·Michael MaceykaSarah Spiegel
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Nov 7, 2006·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Donna L Cioffi, Troy Stevens
Oct 30, 2007·Nature Immunology·Nicole C KaneiderAthan Kuliopulos
Jun 21, 2008·Journal of Cell Science·Elisabetta DejanaMaria Grazia Lampugnani
Sep 12, 2008·Antioxidants & Redox Signaling·Donna L CioffiTroy Stevens
Jan 27, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Raj WadgaonkarViswanathan Natarajan
Sep 15, 2009·American Journal of Respiratory Cell and Molecular Biology·Saad SammaniJoe G N Garcia
Feb 4, 2010·Thrombosis and Haemostasis·A DietrichT Gudermann
Oct 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tetsuro KusabaHiroaki Matsubara
Dec 4, 2010·Antioxidants & Redox Signaling·Donna L Cioffi
Feb 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Libing SongMengfeng Li
May 7, 2011·Nature Reviews. Immunology·Sarah Spiegel, Sheldon Milstien
Jun 30, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Biji MathewJoe G N Garcia
Aug 31, 2011·International Review of Cell and Molecular Biology·Alejandra ChavezDolly Mehta
Oct 18, 2011·Trends in Cell Biology·Michael MaceykaSarah Spiegel
Jun 8, 2012·Sub-cellular Biochemistry·Andrew P Kowalczyk, Benjamin A Nanes
Feb 16, 2013·Circulation Journal : Official Journal of the Japanese Circulation Society·David J Beech
Mar 2, 2013·American Journal of Respiratory Cell and Molecular Biology·Viswanathan NatarajanJoe G N Garcia
Aug 6, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiaoguang SunJoe G N Garcia
Aug 30, 2013·The New England Journal of Medicine·Derek C Angus, Tom van der Poll
Mar 5, 2014·The Journal of Experimental Medicine·Haixia GongAsrar B Malik
May 23, 2014·Journal of Cellular Biochemistry·Xiuqin NiJeffrey R Jacobson
Jul 9, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eugene Y KimSarah Spiegel

❮ Previous
Next ❯

Citations

May 21, 2020·Frontiers in Physiology·Tullio GenovaLuca Munaron
Aug 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nityanand SrivastavaDolly Mehta
Dec 24, 2019·The American Journal of Pathology·Prarthana J DalalDavid P Sullivan
Jun 8, 2019·Drug Discovery Today·Francine Behar-CohenMin Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.