Transient receptor potential melastatin-4 is involved in hypoxia-reoxygenation injury in the cardiomyocytes

PloS One
Hulin PiaoKeiji Naruse

Abstract

Ischemic heart disease still remains the most common cause of cardiac death. During ischemia-reperfusion (I/R), reactive oxygen species (ROS) are produced in excess in cardiac tissue, where they induce cell death. Our previous study showed that 9-phenanthrol (9-Phe), a specific inhibitor of the TRPM4 channel, preserves cardiac contractile function and protects the heart from I/R injury-related infarction in the excised rat heart. Accordingly, we hypothesized that TRPM4 channels are involved in the 9-Phe-mediated cardioprotection against ROS-induced injury. In rats, intravenous 9-Phe mitigated the development of myocardial infarction caused by the occlusion of the left anterior descending artery. Immunohistochemical analysis indicated that TRPM4 proteins are expressed in ventricular myocytes susceptible to I/R injury. Hydrogen peroxide (H2O2) is among the main ROS overproduced during I/R. In the cardiomyocyte cell line H9c2, pretreatment with 9-Phe prevented cell death induced by conditions mimicking I/R, namely 200 μM H2O2 and hypoxia-reoxygenation. Gene silencing of TRPM4 preserved the viability of H9c2 cardiomyocytes exposed to 200 μM H2O2. These results suggest that the cardioprotective effects of 9-Phe are mediated through ...Continue Reading

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Aug 15, 2015·Cardiovascular Research·Romain GuinamardLaurent Sallé
Oct 21, 2015·Seminars in Immunopathology·Tomohiro NumataRyuji Inoue
Mar 25, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qian YuXia Cao
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Jan 1, 2021·Cardiovascular Therapeutics·Mohamed-Yassine Amarouch, Jaouad El Hilaly
Feb 18, 2021·Pflügers Archiv : European journal of physiology·Jianlin FengLixia Yue
Jun 19, 2021·Biochemical and Biophysical Research Communications·Chen WangKen Takahashi
Aug 3, 2021·Frontiers in Pharmacology·Prakash ArullampalamHugues Abriel
Aug 26, 2021·MethodsX·Chen WangKen Takahashi

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Methods Mentioned

BETA
coronary artery bypass
Assay
PCR
Protein Assay
transfection

Software Mentioned

Graphpad
Prism
image J

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