Transient receptor potential vanilloid-1 regulates osteoprotegerin/RANKL homeostasis in human periodontal ligament cells

Journal of Periodontal Research
Sireerat SooamponP Pavasant

Abstract

Increasing evidence has shown the presence of transient receptor potential vanilloid-1 (TRPV1) in a variety of nonneuronal tissues; however, the function of TRPV1 in these cells is not well understood. In this study, we aimed to investigate the expression and function of TRPV1 in human periodontal ligament (HPDL) cells. As HPDL cells are known to play an important role in the bone-remodeling process, we hypothesized that TRPV1 might be implicated in the regulation of osteoprotegerin (OPG) and RANKL expression. TRPV1 expression was examined by western blot analysis. The function of TRPV1 was studied using capsaicin, a well-known TRPV1 agonist. RT-PCR was performed to study the expression of OPG and RANKL mRNAs. The expression of OPG and RANKL proteins was analyzed by ELISA and western blotting, respectively. The mechanisms of capsaicin-induced OPG expression in HPDL cells were studied using inhibitors. In this study we found that TRPV1 was present in HPDL cells. Treatment with capsaicin induced OPG expression in a dose-dependent manner but did not affect the expression of RANKL. The increase of the OPG/RANKL ratio was also found in human osteoblasts, but not in MC3T3-E1 cells, a mouse osteoblastic cell line, suggesting species s...Continue Reading

References

Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·H HsuW J Boyle
Feb 28, 2001·British Journal of Pharmacology·P McIntyreI F James
Aug 2, 2001·Biochemical and Biophysical Research Communications·M DendaK Matsunaga
Aug 6, 2002·Nature Neuroscience·L A BirderM J Caterina
Dec 11, 2002·Journal of Periodontal Research·Tomokazu HasegawaTetsuo Shirakawa
Dec 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Katharine M WalkerPeter McIntyre
Sep 10, 2003·Biochemical Pharmacology·Shinichi KatoKoji Takeuchi
Jan 22, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tracy D Wilson-GerwingValerie M K Verge
Mar 25, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joseph R GhilardiPatrick W Mantyh
Oct 26, 2005·Neuroscience Letters·Luis MenéndezAna Baamonde
May 11, 2006·Biochemical and Biophysical Research Communications·Yan ZhangGuang-Yan Yu
Sep 25, 2007·Journal of Dental Research·H OkaT Takata
Jun 2, 2009·Biochemical Pharmacology·Philip R KymCharles W Hutchins
May 14, 2011·Biochemical and Biophysical Research Communications·Azin AzariTeun J de Vries

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Citations

Jan 13, 2015·Archives of Oral Biology·Ga-Yeon SonDong Min Shin
Sep 17, 2014·PloS One·Burcu ÖzdemirOleh Andrukhov
May 16, 2018·Oral Diseases·Patricia Oliveira de LimaKarina Cogo-Müller
Mar 27, 2020·Frontiers in Oncology·Zhengfeng YangZhenyao Xu

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