Transient receptor potential vanilloid 4 (TRPV4) activation by arachidonic acid requires protein kinase A-mediated phosphorylation

The Journal of Biological Chemistry
Sheng CaoDavid X Zhang

Abstract

Transient receptor potential vanilloid 4 (TRPV4) is a Ca2+-permeable channel of the transient receptor potential (TRP) superfamily activated by diverse stimuli, including warm temperature, mechanical forces, and lipid mediators such as arachidonic acid (AA) and its metabolites. This activation is tightly regulated by protein phosphorylation carried out by various serine/threonine or tyrosine kinases. It remains poorly understood how phosphorylation differentially regulates TRPV4 activation in response to different stimuli. We investigated how TRPV4 activation by AA, an important signaling process in the dilation of coronary arterioles, is affected by protein kinase A (PKA)-mediated phosphorylation at Ser-824. Wildtype and mutant TRPV4 channels were expressed in human coronary artery endothelial cells (HCAECs). AA-induced TRPV4 activation was blunted in the S824A mutant but was enhanced in the phosphomimetic S824E mutant, whereas the channel activation by the synthetic agonist GSK1016790A was not affected. The low level of basal phosphorylation at Ser-824 was robustly increased by the redox signaling molecule hydrogen peroxide (H2O2). The H2O2-induced phosphorylation was accompanied by an enhanced channel activation by AA, and t...Continue Reading

References

Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Nov 30, 2000·FEBS Letters·U WissenbachV Flockerzi
Apr 15, 2003·The Journal of Biological Chemistry·Makoto SuzukiMasashi Imai
Oct 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wolfgang Liedtke, Jeffrey M Friedman
Dec 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·J VriensB Nilius
Jan 7, 2004·American Journal of Physiology. Cell Physiology·Bernd NiliusThomas Voets
Jun 25, 2004·Nucleic Acids Research·David E KimDavid Baker
Mar 9, 2005·The Journal of Cell Biology·Yaniré N AndradeMiguel A Valverde
Oct 14, 2005·Journal of Computational Chemistry·James C PhillipsKlaus Schulten
Nov 5, 2005·Circulation Research·Scott EarleyJoseph E Brayden
Feb 8, 2006·Annual Review of Physiology·I Scott RamseyDavid E Clapham
Apr 7, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicole Alessandri-HaberJon D Levine
May 6, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Ralf KöhlerJoachim Hoyer
Nov 1, 2006·The Journal of General Physiology·Xuming Zhang, Peter A McNaughton
Mar 8, 2007·The Journal of Biological Chemistry·Joris VriensBernd Nilius
Sep 6, 2007·PloS One·Veronika HartmannsgruberRalf Köhler
Nov 6, 2008·Journal of Structural and Functional Genomics·Dmitry RykunovAndrás Fiser
Nov 27, 2008·The Journal of Biological Chemistry·Tomasz WegierskiGerd Walz
Mar 13, 2009·Cell Calcium·Trevor J Shuttleworth
Aug 8, 2009·The Journal of Biological Chemistry·Hueng-Chuen FanPeter A McNaughton
Dec 8, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Suelhem A MendozaDavid X Zhang
Jan 2, 2010·Biochemical and Biophysical Research Communications·Hongquan PengTomasz Wegierski
Jul 8, 2010·The Journal of Biological Chemistry·Stephen LoukinChing Kung
Oct 1, 2010·Journal of Cardiovascular Pharmacology·David X Zhang, David D Gutterman
May 18, 2011·Molecular and Cellular Biology·Theun de GrootJoost G Hoenderop
Dec 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Aaron H BubolzDavid X Zhang
Dec 31, 2011·Journal of Applied Physiology·Tatsuya KusudoHitoshi Yamashita
Aug 8, 2012·Antioxidants & Redox Signaling·Joseph R BurgoynePhilip Eaton
Nov 13, 2012·Journal of Molecular and Cellular Cardiology·Ravi K AdapalaCharles K Thodeti
Jan 12, 2013·EMBO Reports·Bernd Nilius, Thomas Voets

❮ Previous
Next ❯

Citations

Oct 12, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Oleg PalyginAndrey Sorokin
Jan 31, 2020·The Journal of Clinical Investigation·Sandip M SwainRodger A Liddle
Sep 18, 2020·Expert Opinion on Investigational Drugs·Asia Fernández-CarvajalAntonio Ferrer-Montiel
Aug 15, 2019·American Journal of Physiology. Cell Physiology·Stefanie SimpsonBonnie Blazer-Yost
Dec 19, 2018·Frontiers in Physiology·Tarik SmaniJuan A Rosado
Aug 28, 2020·Frontiers in Pharmacology·Anderson Fellyp Avelino DinizBagnólia Araújo da Silva
Dec 11, 2020·The Journal of Biological Chemistry·Sandip M Swain, Rodger A Liddle
Feb 5, 2021·Frontiers in Bioengineering and Biotechnology·Setareh SianatiKate Poole
Feb 19, 2020·Pharmacology & Therapeutics·Boris ManouryRodolphe Fischmeister
Mar 20, 2021·European Journal of Pharmacology·Shiori KawasakiMasahide Fujita
Apr 4, 2021·International Journal of Molecular Sciences·Karolina Nowicka-Bauer, Monika Szymczak-Cendlak
Feb 20, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Rebecca L ShawSteven S Segal
May 14, 2021·Frontiers in Molecular Biosciences·Liangliang LiuTingting Zhou
Oct 21, 2021·Cardiovascular Research·Benjamin GrimmerWolfgang M Kuebler

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.