Transient receptor potential vanilloid 4 regulates aquaporin-5 abundance under hypotonic conditions

Proceedings of the National Academy of Sciences of the United States of America
Venkataramana K SidhayeLandon S King

Abstract

Aquaporin-5 (AQP5) is expressed in epithelia of lung, cornea, and various secretory glands, sites where extracellular osmolality is known to fluctuate. Hypertonic aquaporin (AQP) induction has been described, but little is known about the effects of a hypotonic environment on AQP abundance. We report that, when mouse lung epithelial cells were exposed to hypotonic medium, a dose-responsive decrease in AQP5 abundance was observed. Hypotonic reduction of AQP5 was blocked by ruthenium red, methanandamide, and miconazole, agents that inhibit the cation channel transient receptor potential vanilloid (TRPV) 4 present in lung epithelial cells. Several observations indicate that TRPV4 participates in hypotonic reduction of AQP5, including a requirement for extracellular calcium to achieve AQP5 reduction; an increase in intracellular calcium in mouse lung epithelial (MLE) cells after hypotonic stimulation; and reduction of AQP5 abundance after addition of the TRPV4 agonist 4alpha-Phorbol-12,13-didecanoate (4alpha-PDD). Similarly, addition of hypotonic PBS to mouse trachea in vivo decreased AQP5 within 1 h, an effect blocked by ruthenium red. To confirm a functional interaction, AQP5 was expressed in control or TRPV4-expressing human emb...Continue Reading

References

Mar 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·R C BoucherJ T Gatzy
Dec 17, 1981·The New England Journal of Medicine·M KnowlesR Boucher
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K A WikenheiserJ A Whitsett
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·L G DobbsA S Verkman
Dec 30, 1999·American Journal of Respiratory Cell and Molecular Biology·J E TowneA G Menon
Mar 18, 2000·The Journal of Biological Chemistry·J D HoffertL S King
May 17, 2000·The Journal of Clinical Investigation·H MatsuiR C Boucher
Sep 13, 2000·The Journal of Allergy and Clinical Immunology·S D Anderson, K Holzer
Nov 30, 2000·FEBS Letters·U WissenbachV Flockerzi
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·V LeitchL S King
Mar 10, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·S SteinfeldC Delporte
Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·S M KredaS E Gabriel
Mar 30, 2001·The Journal of Biological Chemistry·J E TowneA G Menon
May 2, 2001·The Journal of General Physiology·S JayaramanA S Verkman
Jun 13, 2001·American Journal of Physiology. Cell Physiology·T MatsuzakiK Takata
Aug 22, 2001·The Journal of Biological Chemistry·Y Song, A S Verkman
Dec 26, 2001·Biochimica Et Biophysica Acta·Y SugiyamaS Inoue
Feb 6, 2002·The Journal of Biological Chemistry·Hiroyuki WatanabeBernd Nilius
Apr 5, 2002·Trends in Pharmacological Sciences·Martin J GunthorpeJohn B Davis
Feb 5, 2003·Experimental Eye Research·A S Verkman
Jan 7, 2004·American Journal of Physiology. Cell Physiology·Bernd NiliusThomas Voets
Apr 13, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yanlin JiaJohn A Hey
Jul 13, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Wolfgang Liedtke, S A Simon
Aug 4, 2004·Micron : the International Research and Review Journal for Microscopy·Harriet NilssonGodfried M Roomans
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Landon S KingPeter Agre
Sep 14, 2004·Journal of Neurobiology·Makoto Tominaga, Michael J Caterina
Oct 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andreas HerrlichLandon S King
Nov 13, 2004·The Journal of Biological Chemistry·Venkataramana SidhayeLandon S King
Dec 25, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Inna KozlovaGodfried M Roomans
Dec 29, 2004·FEBS Letters·Stefan KippenbergerAugust Bernd
Jan 14, 2005·Journal of the American Society of Nephrology : JASN·Fuminori UmenishiRobert W Schrier
Mar 9, 2005·The Journal of Cell Biology·Yaniré N AndradeMiguel A Valverde

❮ Previous
Next ❯

Citations

Feb 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Venkataramana K SidhayeLandon S King
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Valentina BenfenatiMahmood Amiry-Moghaddam
Mar 13, 2009·Human Molecular Genetics·Guohua ZhuSreekumar G Pillai
Jun 21, 2012·PloS One·Venkataramana K SidhayeLandon S King
Jun 5, 2007·The Journal of Membrane Biology·Adrian CaplanusiDanny Jans
Feb 18, 2014·Molecular Neurobiology·Yuanzheng Gu, Chen Gu
Jan 12, 2016·Channels·Ana Galán-CoboMiriam Echevarría
Oct 20, 2009·Progress in Biophysics and Molecular Biology·Wouter EveraertsGrzegorz Owsianik
Dec 17, 2008·Annals of the New York Academy of Sciences·Wolfgang Liedtke
Apr 19, 2011·Anatomia, histologia, embryologia·M YangS Q Sun
Nov 6, 2015·Pflügers Archiv : European journal of physiology·Kazuo Hosoi
Feb 11, 2010·The Journal of Obstetrics and Gynaecology Research·Hongbo QiJin Huang
Sep 17, 2013·Basic & Clinical Pharmacology & Toxicology·Lisa AlenmyrPeter M Zygmunt
Nov 21, 2007·Biochemical and Biophysical Research Communications·Su Wang, Yuchun Gu
Oct 5, 2013·Biochimica Et Biophysica Acta·Rebecca E DayMatthew T Conner
Nov 17, 2015·PloS One·Philip KitchenSusanna Törnroth-Horsefield
Aug 11, 2016·Journal of Anesthesia·Koji Shibasaki
Nov 27, 2008·The Journal of Biological Chemistry·Tomasz WegierskiGerd Walz
Aug 20, 2011·Journal of the American Society of Nephrology : JASN·Stine Falsig PedersenElse K Hoffmann
Jan 24, 2007·Physiological Reviews·Bernd NiliusJohn A Peters
Jun 15, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Marjoke HeneweerAd Peijnenburg
Jan 5, 2008·The Journal of Biological Chemistry·Dieter D'hoedtBernd Nilius
Jul 9, 2008·Acta Oto-laryngologica·Takuya IshibashiMatti Anniko
Feb 13, 2009·American Journal of Physiology. Cell Physiology·Udo Hasler
Sep 6, 2011·Pflügers Archiv : European journal of physiology·Damien S K Samways, Terrance M Egan
Dec 31, 2011·Journal of Applied Physiology·Tatsuya KusudoHitoshi Yamashita
Feb 11, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yasunori TakayamaMakoto Tominaga
Aug 9, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Clémence O HenryMustapha Si-Tahar
Jun 20, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tamara Hew-ButlerJoseph G Verbalis
Jan 20, 2007·Biochemical Society Transactions·C Harteneck, B Reiter
Jan 23, 2018·Frontiers in Immunology·Jürg HamacherUz Stammberger
Oct 11, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Karthik SureshLarissa A Shimoda
Jan 4, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Koji Shibasaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.