Transient reperfusion with acidic solution affects postischemic functional recovery: studies in the isolated working rat heart

The Journal of Thoracic and Cardiovascular Surgery
T OhashiY Kawashima

Abstract

This isolated working rat heart study was designed to investigate the effect of duration of reperfusion and degree of acidity of the reperfusate on myocardial protection. The experimental time course was as follows: 20 minutes of perfusion with the heart working, 3 minutes of infusion with the St. Thomas' Hospital cardioplegic solution followed by global ischemia for 33 minutes at 37 degrees C, and 20 minutes of Langendorff reperfusion followed by 20 minutes of working perfusion. During the initial 3 minutes of Langendorff reperfusion, the pH of the reperfusate was changed to 5.6, 6.8, and 7.5 by addition of sodium hydroxide into Krebs-Henseleit nonbicarbonate HEPES buffer. A respiratory acidic reperfusate was used for the initial 0.5, 1, 2, 3, 5, and 15 minutes during reperfusion. The results were as follows: (1) Reperfusion with a mildly acidic solution (i.e., pH 6.8) yielded better recovery than reperfusion with solutions having pH levels of 5.8 or 7.5. (2) Reperfusion for less than 3 minutes with a reperfusate having a pH level of 6.8 provided better recovery, although reperfusion for longer than 3 minutes exacerbated reperfusion injury. In conclusion, the effects of reperfusion with acidic solution were influenced by degre...Continue Reading

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Citations

May 16, 2009·Basic Research in Cardiology·Antonio Rodríguez-SinovasDavid García-Dorado
Jul 24, 2013·General Thoracic and Cardiovascular Surgery·Hiroshi Yamamoto, Fumio Yamamoto
Jun 29, 2010·Antioxidants & Redox Signaling·Javier InserteDavid Garcia-Dorado
Dec 7, 2007·Cardiovascular Research·Javier InserteDavid Garcia-Dorado
Oct 10, 2014·British Journal of Pharmacology·Javier Inserte, David Garcia-Dorado
Apr 16, 1998·The Journal of Thoracic and Cardiovascular Surgery·F P TrittoJ Soler-Soler
Sep 1, 2015·Frontiers in Neuroscience·Ornella CuomoGiuseppe Pignataro
Oct 22, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Diego LópezDavid García-Dorado

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