Transient stability of the helical pattern of region F19-L22 of the N-terminal domain of p53: a molecular dynamics simulation study

Biochemical and Biophysical Research Communications
L M Espinoza-Fonseca, José G Trujillo-Ferrara

Abstract

Two molecular dynamics simulations of the region E17-N29 of p53 (p53(17-29)) at different temperatures were performed for a total time of 0.2 micros, to study the conformational landscape of this region. Previous studies have suggested that this region displays different structural motifs, such as helix of a double beta-turn, and that its secondary structure might be transiently stable. Interestingly, in this study it was found that the region F19-L25, and particularly its fragment F19-L22, display a stable, transient helical pattern at sub-microsecond periods. The region F19-L22, which contains one of the most important residues needed for the interaction of p53 with MDM2, seems to be formed and stabilized by the existence of one hydrophobic and one aromatic cluster. The main function of these clusters is to help their surrounding area to desolvate, to allow the hydrogen bond network, therefore favoring the formation of a stable helix. This preliminary study would be useful for a better understanding of the structure and function of the N-terminal domain of p53 and its implications for the control of different types of cancer.

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Citations

Mar 3, 2010·BMC Genomics·Jagadeesh N MavinahalliChandra Verma
May 5, 2011·International Journal of Molecular Sciences·Yongqi Huang, Zhirong Liu
Sep 6, 2012·International Journal of Molecular Sciences·Ting FuGuohui Li
Jan 1, 2015·Progress in Biophysics and Molecular Biology·Taniya SahaGaurisankar Sa
Aug 27, 2013·Cell Cycle·Giovanni ChillemiGerry Melino
Jun 6, 2015·PLoS Computational Biology·Juan A Bueren-Calabuig, Julien Michel
Jul 4, 2006·Biopolymers·L M Espinoza-Fonseca, José G Trujillo-Ferrara
Feb 7, 2019·Journal of Molecular Cell Biology·Yaw Sing TanChandra S Verma
Mar 28, 2009·Journal of Chemical Information and Modeling·Ori Kalid, Nir Ben-Tal
Dec 4, 2013·The Journal of Physical Chemistry. B·Elio A CinoMikko Karttunen

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