Transient-state kinetic analysis of the oxidative decarboxylation of D-malate catalyzed by tartrate dehydrogenase

Biochemistry
P A Tipton

Abstract

The oxidative decarboxylation of D-malate catalyzed by tartrate dehydrogenase has been analyzed by transient-state kinetic methods and kinetic isotope effect measurements. The reaction time courses show a burst of NADH formation prior to the attainment of the steady-state velocity. The binding of the inhibitor tartronate to the enzyme was examined by monitoring the quenching of the protein's intrinsic fluorescence; the tartronate concentration dependence of the observed rate constant for association was hyperbolic, supporting a two-step model for inhibitor binding. Analysis of the time courses for D-malate oxidation yielded values for many of the microscopic rate constants governing the reaction. The range of possible solutions for the microscopic rate constants was constrained by comparison of the time course for oxidation of unlabeled malate with that of deuterated malate; this analysis relied on the determination of the intrinsic isotope effect on hydride transfer via measurement of D(V/K), T(V/K), and the oxaloacetate partition ratio. The results of the transient-state kinetic analyses suggest that the rate of D-malate oxidation is largely limited by the rate of decarboxylation of the intermediate oxaloacetate which occurs ...Continue Reading

References

Mar 1, 1989·The Biochemical Journal·C T Zimmerle, C Frieden
Aug 15, 1994·Archives of Biochemistry and Biophysics·P A Tipton, B S Beecher

❮ Previous
Next ❯

Citations

Jun 3, 2010·Acta Crystallographica. Section D, Biological Crystallography·Radhika Malik, Ronald E Viola

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.