PMID: 9523728Apr 2, 1998Paper

Transient up-regulation of a prolyl endopeptidase activity in the microsomal fraction of rat liver during postnatal development

European Journal of Biochemistry
Y MatsubaraS Kawashima

Abstract

To identify proteases which are involved in cell proliferation and differentiation of liver cells, we assayed various protease activities in rat liver during its postnatal development. We found that a protease activity specific to proline residues in the microsomal fraction of rat liver was transiently increased around postnatal day 8 and decreased thereafter, indicating that the enzyme activity is highly correlated to the proliferation and differentiation of liver cells. This protease was purified to an apparent homogeneity from the microsomal fraction of the postnatal-day-8 rat liver. The purified enzyme gave a single band with an apparent molecular mass of 65,000 on SDS/PAGE. It cleaved several peptide 4-methylcoumaryl-7-amide substrates and bioactive peptides at the C-terminus of proline residues. The enzyme did not hydrolyze any protein substrate examined suggesting that it is a peptidase rather than a proteinase. Although the best substrate among those tested was succinyl-Gly-Pro-Leu-Gly-Pro-4-methylcoumaryl-7-amide (-NH-Mec), the purified enzyme did not hydrolyze succinyl-Gly-Pro-NH-Mec, indicating that the enzyme has different substrate specificity from any hitherto known prolyl endopeptidases. These results suggest tha...Continue Reading

Citations

Jul 12, 2008·Histochemistry and Cell Biology·Timo T MyöhänenPekka T Männistö
May 6, 2011·Journal of Molecular Histology·Shin MatsubaraAtsushi P Kimura
Aug 19, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Timo T MyöhänenPekka T Männistö
Jan 2, 2007·Neuropeptides·J A García-HorsmanJ I Venäläinen
Nov 25, 2006·Regulatory Peptides·Naiara AgirregoitiaJon Irazusta
Sep 4, 2015·Journal of Cellular Physiology·Raffaele DotoloSabrina Diano
May 31, 2011·Biochemical and Biophysical Research Communications·Minoru SakaguchiMasanori Takaoka
Nov 1, 2018·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Massimo Venditti, Sergio Minucci
Apr 17, 2019·Molecular Reproduction and Development·Ping XuChunping Zhang
May 8, 2019·Zygote : the Biology of Gametes and Early Embryos·Massimo VendittiSergio Minucci
Aug 14, 1999·The Journal of Biological Chemistry·A KimuraT Takahashi
Mar 30, 2017·Experimental and Therapeutic Medicine·Da ZhouJian-Gao Fan

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