Transition from Hybrid Capture 2 to Cobas 4800 in Hpv detection: sensitivity and specificity for Cin2+ in two time periods

Infectious Diseases
Fabio BottariMaria Teresa Sandri

Abstract

High-risk (HR) Human Papilloma Virus (HPV) Tests for HPV detection differ in sensitivity and specificity. In this study, we evaluated the sensitivity and specificity of the HC2 HR HPV Test and the Cobas 4800 HPV Test in consecutive cervical samples collected from a referral population with a high prevalence of disease, using CIN2+ histology as clinical outcome. Ten thousand two-hundred and thirteen consecutive cervical samples were assayed for HR-HPV in the Laboratory Medicine Division of IEO: 5140 from January 2012 to June 2013 with HC2 and 5073 from July 2013 to December 2014 with the Cobas HPV Test. These two assays differ in terms of target genes and testing methods. The test positivity rates for HC2 and Cobas 4800 were 29.5% (1515/5135, 95% CI 28.3-30.8%) and 23.9% (1212/5069, 95% CI 22.7-25.1%), respectively. The detection rates of CIN2+ in the two time periods were 2.8% (145/5140, 95% CI 2.4-3.3%) and 1.6% (79/5073, 95% CI 1.2-1.9%), respectively. The sensitivity for CIN2+ for HC2 and Cobas 4800 was 95.2% (138/145, 95% CI 91.7-98.7%) and 93.7% (74/79, 95% CI 88.3-99.0%), respectively. The specificity for CIN2+ for HC2 and Cobas 4800 was 72.4% (3613/4990, 95% CI 71.2-73.6%) and 77.2% (3852/4990, 95% CI 76.0-78.4%), respec...Continue Reading

References

Sep 1, 2007·Journal of Cellular and Molecular Medicine·C E DepuydtJ J Bogers
Nov 1, 2008·International Journal of Cancer. Journal International Du Cancer·Chris J L M MeijerPeter J F Snijders
Feb 26, 2011·American Journal of Clinical Pathology·Mark H StolerUNKNOWN ATHENA (Addressing THE Need for Advanced HPV Diagnostics) HPV Study Group
Sep 2, 2011·Journal of Clinical Microbiology·D A M HeidemanP J F Snijders
Sep 29, 2011·American Journal of Obstetrics and Gynecology·Thomas C WrightTeresa L Wright
Nov 15, 2011·The Journal of Molecular Diagnostics : JMD·Maria Luisa Mateos LindemannPhilippe Halfon
Mar 14, 2013·PloS One·Belén LloverasSergi Serrano
Aug 13, 2013·European Journal of Obstetrics, Gynecology, and Reproductive Biology·W A A Tjalma, C E Depuydt
Aug 21, 2013·Journal of Lower Genital Tract Disease·Sarah IgidbashianMario Sideri
Jun 20, 2015·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Mark SchiffmanPhilip E Castle
Nov 26, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mario PoljakKatja Seme
Feb 7, 2016·International Journal of Cancer. Journal International Du Cancer·Karl Ulrich PetryCatherine M Behrens
Mar 19, 2016·Cytopathology : Official Journal of the British Society for Clinical Cytology·D FornariJ Bonde
Jun 1, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L de ThurahM Rebolj

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