PMID: 11607326Oct 1, 1992Paper

Transition metals in legume root nodules: iron-dependent free radical production increases during nodule senescence

Proceedings of the National Academy of Sciences of the United States of America
Manuel Becana, Robert V Klucas

Abstract

The cytosol from root nodules of soybean, bean, and cowpea contained Fe and Cu capable of catalyzing the formation of highly reactive free radicals. Specific and sensitive assays based on free radical-mediated DNA degradation revealed that most catalytic Fe and Cu were present as small chelates (300-600 Da). The involvement of catalytic Fe in free radical production during nodule senescence, which was induced by exposure of plants to continuous darkness for 2-4 days, was investigated. (i) Free heme remained at a constant and low concentration (1-4% of total nodule heme) during senescence, indicating that it is not an important constituent of the catalytic Fe pool of nodules. (ii) Catalytic Fe of nodule cytosol promoted deoxyribose degradation and linolenic acid peroxidation in reaction mixtures containing physiological concentrations of ascorbate and H2O2. Deoxyribose degradation but not lipid peroxidation required hydroxyl radicals to proceed. (iii) The cytosol from senescent nodules, particularly of bean and cowpea, sustained in vitro higher rates of deoxyribose degradation and lipid peroxidation than the cytosol from unstressed nodules. Both degradative processes were inhibited by the Fe chelator desferrioxamine and were cor...Continue Reading

References

Jan 1, 1979·Analytical Biochemistry·T A LaRue, J J Child
May 15, 1989·Archives of Biochemistry and Biophysics·D M Miller, S D Aust
Jun 1, 1984·Analytical Biochemistry·B D PattersonI B Ferguson
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Becana, R V Klucas
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D A DaltonH J Evans
Aug 1, 1989·Plant Physiology·C F BabbsR C Coolbaugh

❮ Previous
Next ❯

Citations

Nov 10, 2007·Molecular Plant-microbe Interactions : MPMI·Catrin GüntherThomas Ott
Feb 19, 2014·Archives of Environmental Contamination and Toxicology·Sumit K SoniAlok Kalra
Oct 9, 2014·Journal of Experimental Botany·Siân L RichardsJulia M Davies
Feb 16, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Eunae ChoSeunho Jung
Apr 11, 2009·Journal of Theoretical Biology·Diana E MarcoJuan Sanjuán
Oct 27, 2015·Journal of Photochemistry and Photobiology. B, Biology·Heta MattilaEsa Tyystjärvi
Nov 3, 2010·The New Phytologist·Manuel BecanaDavid A Dalton
May 9, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Brahma N SinghH B Singh
Jul 27, 1999·Journal of Bacteriology·R SantosD Touati
Nov 29, 2002·Journal of Cell Science·Vadim DemidchikJulia M Davies
Oct 14, 2003·Plant Physiology·Manuel A MatamorosManuel Becana

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.