Transition of aortic endothelial cells from resting to migrating cells is associated with three sequential patterns of microfilament organization

Journal of Vascular Research
T Y LeeA I Gotlieb

Abstract

The endothelial cell is unique because it must undergo a transition from a resting cell with a cytoskeleton organized for barrier function to one which promotes cell translocation following denuding endothelial injury. Since actin microfilaments are critical for both maintaining the integrity of the resting monolayer and for optimum reendothelialization, we carried out a detailed study of the organization of microfilaments as the cell undergoes the transition from a resting to a translocating cell. We used an in vitro model in which a linear wound was made in a confluent monolayer of porcine aortic endothelial cells. The complex reorganization of actin microfilament bundles following injury and their relationship to microtubules and vinculin was studied in cells at the wound edge using immunofluorescent scanning laser confocal microscopy and time-lapse videomicroscopy. In the resting confluent monolayer, microfilaments were present as a dense peripheral band (DPB) located toward the upper part of the cell and as central microfilament bundles at the substratum. Three distinct stages of microfilament reorganization occurred sequentially during early repair. Stage 1 followed wounding and involved the reduction of the DPBs of micro...Continue Reading

Citations

Apr 6, 2002·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Adam D Durbin, Avrum I Gotlieb
Jun 19, 2004·Endothelium : Journal of Endothelial Cell Research·Amir RezvanPeter I Lelkes
Feb 2, 2000·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·W D EhringerF N Miller
Jan 28, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·S ColangeloA I Gotlieb
Mar 12, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·T A Akong, A I Gotlieb
Aug 23, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dmitry KondrikovYunchao Su
Sep 21, 2006·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Anders SondénB Thomas Kjellström
Dec 18, 2002·Experimental Biology and Medicine·Maria Luiza C Albuquerque, Annette S Flozak
Nov 7, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R S PiotrowiczE G Levin
Jul 19, 2000·American Journal of Physiology. Heart and Circulatory Physiology·M L AlbuquerqueA S Flozak

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