Transition zone assembly and its contribution to axoneme formation in Drosophila male germ cells

The Journal of Cell Biology
Jennifer VieillardBénédicte Durand


The ciliary transition zone (TZ) is a complex structure found at the cilia base. Defects in TZ assembly are associated with human ciliopathies. In most eukaryotes, three protein complexes (CEP290, NPHP, and MKS) cooperate to build the TZ. We show that in Drosophila melanogaster, mild TZ defects are observed in the absence of MKS components. In contrast, Cby and Azi1 cooperate to build the TZ by acting upstream of Cep290 and MKS components. Without Cby and Azi1, centrioles fail to form the TZ, precluding sensory cilia assembly, and no ciliary membrane cap associated with sperm ciliogenesis is made. This ciliary cap is critical to recruit the tubulin-depolymerizing kinesin Klp59D, required for regulation of axonemal growth. Our results show that Drosophila TZ assembly in sensory neurons and male germ cells involves cooperative actions of Cby and Dila. They further reveal that temporal control of membrane cap assembly by TZ components and microtubule elongation by kinesin-13 is required for axoneme formation in male germ cells.


Feb 1, 1970·The Journal of Cell Biology·D M Phillips
Oct 3, 2003·Current Biology : CB·Young-Goo HanMaurice J Kernan
May 26, 2006·Annual Review of Genomics and Human Genetics·Jose L BadanoNicholas Katsanis
Sep 22, 2006·Journal of Cell Science·Carolyn A Moores, Ronald A Milligan
Apr 17, 2007·Current Biology : CB·Christine BlaineauPatrick Bastien
Sep 2, 2008·Genetics·Juan HuangYang Hong
Apr 15, 2009·The Journal of Cell Biology·Vera A VoroninaRandall T Moon
Jul 15, 2009·Seminars in Cell & Developmental Biology·Delphine Gogendeau, Renata Basto
Oct 31, 2009·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Kate Baker, Philip L Beales
Aug 24, 2010·Molecular and Biochemical Parasitology·Kuan Yoow Chan, Klaus Ersfeld
May 10, 2011·Current Biology : CB·Tatsuhiko NoguchiShigeo Hayashi
Jun 3, 2011·Cytoskeleton·Qicong Hu, W James Nelson
Jul 5, 2011·Nature Genetics·Francesc R Garcia-GonzaloJeremy F Reiter
Mar 10, 2012·Trends in Cell Biology·Peter G Czarnecki, Jagesh V Shah
Apr 18, 2012·The Journal of Cell Biology·Camille EnjolrasBénédicte Durand
Aug 18, 2012·Developmental Cell·Maria Giovanna RiparbelliTimothy L Megraw
Oct 23, 2012·Spermatogenesis·Lacramioara Fabian, Julie A Brill
Jan 29, 2013·Cilia·Katarzyna Szymanska, Colin A Johnson
Feb 19, 2013·Journal of Cell Science·Liang WangJunmin Pan
Oct 10, 2013·Journal of Cell Science·Marco GottardoMaria Giovanna Riparbelli
Oct 15, 2013·The EMBO Journal·Bine H VillumsenSimon Bekker-Jensen
Nov 5, 2013·Current Biology : CB·Joseph E KlebbaGregory C Rogers
Nov 19, 2013·Biology Open·Maria Giovanna RiparbelliTimothy L Megraw
Dec 4, 2013·Nature Cell Biology·Ching-Hwa Sung, Michel R Leroux
Dec 4, 2013·The Journal of Cell Biology·Anna FranzJordan W Raff
Oct 15, 2014·The Journal of Cell Biology·Michael C BurkeKen-Ichi Takemaru
Dec 3, 2014·Current Biology : CB·Marcus L BasiriTomer Avidor-Reiss
Dec 17, 2014·Molecular Biology of the Cell·Krishna Kumar VasudevanJacek Gaertig
Apr 4, 2015·Methods in Cell Biology·Jennifer VieillardBénédicte Durand
Apr 18, 2015·Frontiers in Cell and Developmental Biology·Tomer Avidor-ReissKyoung H Jo
May 6, 2015·Developmental Cell·Sebastian DunstMarko Brankatschk
May 12, 2015·Bioscience·Jason M Brown, George B Witman
Jul 1, 2015·The Journal of Cell Biology·Clementine SchoutedenAlexander Dammermann
Dec 15, 2015·Current Biology : CB·Tomer Avidor-Reiss, Michel R Leroux
Mar 17, 2016·PLoS Biology·Chunmei LiMichel R Leroux
Jul 7, 2016·Cilia·Swadhin Chandra JanaTimothy L Megraw


Apr 13, 2017·Molecules and Cells·João Gonçalves, Laurence Pelletier
Jan 3, 2019·Molecular Biology of the Cell·Céline AugièreBénédicte Durand
May 28, 2020·International Journal of Molecular Sciences·Maria E TevesPaulene Sapao
Jun 4, 2020·Biochemical Society Transactions·Jean-André LapartBénédicte Durand
May 11, 2018·The Journal of Cell Biology·Petra Zur LageAndrew P Jarman
Sep 10, 2019·Frontiers in Cell and Developmental Biology·Veronica PersicoMaria Giovanna Riparbelli
Jun 9, 2020·Frontiers in Cell and Developmental Biology·Yanan HouQing Wei
Jul 18, 2018·Nature Cell Biology·Swadhin Chandra JanaMónica Bettencourt-Dias
Apr 19, 2019·BMC Biology·Liliane AbuinRichard Benton
Apr 16, 2017·The Journal of Cell Biology·Daniel SerwasAlexander Dammermann
Jul 25, 2020·The Journal of Cell Biology·Jaclyn M Fingerhut, Yukiko M Yamashita

Related Concepts

Drosophila melanogaster
Y-Chromosome-Bearing Sperm
Drosophila melanogaster Proteins

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.