Transitions between the steps of forward and reverse splicing of group IIC introns

RNA
Claire M Smathers, Aaron R Robart

Abstract

Group II introns are mobile genetic elements that perform both self-splicing and intron mobility reactions. These ribozymes are comprised of a catalytic RNA core that binds to an intron-encoded protein (IEP) to form a ribonucleoprotein (RNP) complex. Splicing proceeds through two competing reactions: hydrolysis or branching. Group IIC intron ribozymes have a minimal RNA architecture, and splice almost exclusively through hydrolysis in ribozyme reactions. Addition of the IEP allows the splicing reaction to form branched lariat RNPs capable of intron mobility. Here we examine ribozyme splicing, IEP-dependent splicing, and mobility reactions of a group IIC intron from the thermophilic bacterium Thermoanerobacter italicus (Ta.it.I1). We show that Ta.it.I1 is highly active for ribozyme activity, forming linear hydrolytic intron products. Addition of purified IEP switches activity to the canonical lariat forming splicing reaction. We demonstrate that the Ta.it.I1 group IIC intron coordinates the progression of the forward splicing reaction through a π-π' interaction between intron domains II and VI. We further show that branched splicing is supported in the absence of the IEP when the π-π' interaction is mutated. We also investigated...Continue Reading

References

Jan 1, 1987·Cold Spring Harbor Symposia on Quantitative Biology·C L PeeblesP S Perlman
Jun 1, 1988·Molecular and Cellular Biology·K A JarrellP S Perlman
Jan 31, 1986·Cell·C L PeeblesH L Cheng
May 1, 1995·Molecular and Cellular Biology·J V MoranP S Perlman
Jul 22, 1993·Nature·J L Ferat, F Michel
Jul 17, 1998·Current Opinion in Structural Biology·P Z Qin, A M Pyle
Feb 27, 2001·Nucleic Acids Research·S Zimmerly Wu Xc
Dec 18, 2001·The EMBO Journal·M MatsuuraA M Lambowitz
Apr 2, 2003·Molecular Cell·Yasunori AizawaAnna Marie Pyle
Dec 1, 2004·Annual Review of Genetics·Alan M Lambowitz, Steven Zimmerly
May 26, 2005·Protein Expression and Purification·F William Studier
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Aaron R RobartSteven Zimmerly
Jun 16, 2007·Biological Chemistry·Olga Fedorova, Nora Zingler
Apr 5, 2008·Science·Navtej ToorAnna Marie Pyle
Sep 5, 2008·PloS One·Marta MastroianniAlan M Lambowitz
Mar 21, 2009·Trends in Biochemical Sciences·François MichelEric Westhof
May 16, 2009·Current Opinion in Structural Biology·Navtej ToorAnna Marie Pyle
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Oct 30, 2012·Cell·Marco Marcia, Anna Marie Pyle
Sep 26, 2014·Nature·Aaron R RobartNavtej Toor
May 12, 2015·Mobile DNA·Steven Zimmerly, Cameron Semper
Jun 25, 2015·Microbiology Spectrum·Alan M Lambowitz, Marlene Belfort
May 3, 2016·Nature Structural & Molecular Biology·Guosheng QuHong-Wei Wang
Dec 10, 2016·Science·Maria CostaFrançois Michel
Jun 27, 2017·Methods : a Companion to Methods in Enzymology·Timothy Wiryaman, Navtej Toor
Nov 10, 2018·Nature Communications·Russell T ChanNavtej Toor
May 28, 2019·Nucleic Acids Research·Fernando M García-RodríguezNicolás Toro
Jul 28, 2019·Cell·Daniel B HaackNavtej Toor

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Citations

Jul 17, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Amit Kumar RaiVenkata Naga Srikanth Garikipati

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