Oct 24, 2018

Transitions in cell potency during early mouse development are driven by Notch

BioRxiv : the Preprint Server for Biology
Sergio MencheroMiguel Manzanares

Abstract

The Notch signalling pathway plays fundamental roles in diverse developmental processes in metazoans, where it is important in driving cell fate and directing differentiation of various cell types. However, we still have limited knowledge about the role of Notch in early preimplantation stages of mammalian development, or how it interacts with other signalling pathways active at these stages such as Hippo. By using genetic and pharmacological tools in vivo, together with image analysis of single embryos and pluripotent cell culture, we have found that Notch is active from the 4-cell stage. Transcriptomic analysis in single morula identified novel Notch targets, such as early naive pluripotency markers or transcriptional repressors such as TLE4. Our results reveal a previously undescribed role for Notch in driving transitions during the gradual loss of potency that takes place in the early mouse embryo prior to the first lineage decisions.

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Mentioned in this Paper

Embryo
Regulation of Cell-cell Adhesion
In Vivo
Biochemical Pathway
Laboratory Procedures
Cell Fate
TLE4 gene
Pluripotent Stem Cells
Notch
Entire Embryo

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