PMID: 8938710Jan 1, 1996Paper

Transitions near the open state in Shaker K(+)-channel: probing with temperature

Neuropharmacology
B M Rodríguez, Francisco Bezanilla

Abstract

Ionic currents from non-inactivating Shaker K(+)-channels were expressed in Xenopus oocytes and recorded with the cut-open oocyte voltage clamp technique. Kinetic properties of ionic currents were studied between 1.4 and 19 degrees C. The forward step of the last transition (...C --> O) of the activation pathway, just before the channel opening, was studied by the recovery of the ionic current after a short interval (< 500 microseconds) at a hyperpolarized potential (-90 mV) between two identical depolarizing pulses (0, 40 or 100 mV). Ionic currents under these experimental conditions are described by at least three kinetic components. The predominant kinetic component (corresponding to the ...C --> O transition) has a low temperature dependence (activation energy, Ea, around -5 kcal/mol) and it is voltage independent, suggesting that this step does not carry charge and has a mainly entropic energy barrier. The backward step (O --> C...) was studied from ionic tail currents at -120 mV after pulsing to 40 mV. The closure of the channel has a high temperature dependence (Q10 > 4), having an Ea of 24-25 kcal/mol. The closure of the channel also is voltage dependent, having a valence times fraction of the electric field, z beta of ...Continue Reading

References

Nov 1, 1977·The Journal of General Physiology·F Bezanilla, C M Armstrong
Apr 1, 1985·The Journal of General Physiology·M M White, F Bezanilla
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Apr 1, 1994·Biophysical Journal·E StefaniF Bezanilla
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Feb 1, 1994·The Journal of General Physiology·W N ZagottaR W Aldrich

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Citations

Sep 7, 2000·Biochemical and Biophysical Research Communications·M H WangH I Rhee
Aug 5, 1998·The Journal of General Physiology·B M RodríguezF Bezanilla
Jan 28, 2004·The Journal of General Physiology·Ulrike Laitko, Catherine E Morris
Dec 30, 2004·The Journal of General Physiology·Alain J LabroDirk J Snyders
Nov 28, 2012·The Journal of General Physiology·Ernesto VargasBenoît Roux
Oct 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sebastian BrauchiRamon Latorre
Aug 27, 2014·Cell·Sandipan ChowdhuryBaron Chanda
Feb 3, 2006·American Journal of Physiology. Cell Physiology·Jamie I VandenbergChristopher L-H Huang
Apr 4, 2000·Physiological Reviews·F Bezanilla
Apr 5, 2001·American Journal of Physiology. Cell Physiology·J Li, A M Correa
Oct 1, 1998·The American Journal of Physiology·A M Correa
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Thomas C RichDirk J Snyders
Oct 18, 2020·International Journal of Molecular Sciences·Juan ZhaoRikard Blunck
Apr 14, 2012·Science·Morten Ø JensenDavid E Shaw

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