Translation elongation and mRNA stability are coupled through the ribosomal A-site

RNA
Gavin HansonJeff Coller

Abstract

Messenger RNA (mRNA) degradation plays a critical role in regulating transcript levels in eukaryotic cells. Previous work by us and others has shown that codon identity exerts a powerful influence on mRNA stability. In Saccharomyces cerevisiae, studies using a handful of reporter mRNAs show that optimal codons increase translation elongation rate, which in turn increases mRNA stability. However, a direct relationship between elongation rate and mRNA stability has not been established across the entire yeast transcriptome. In addition, there is evidence from work in higher eukaryotes that amino acid identity influences mRNA stability, raising the question as to whether the impact of translation elongation on mRNA decay is at the level of tRNA decoding, amino acid incorporation, or some combination of each. To address these questions, we performed ribosome profiling of wild-type yeast. In good agreement with other studies, our data showed faster codon-specific elongation over optimal codons and faster transcript-level elongation correlating with transcript optimality. At both the codon-level and transcript-level, faster elongation correlated with increased mRNA stability. These findings were reinforced by showing increased transl...Continue Reading

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Citations

Dec 7, 2018·The Journal of Biological Chemistry·Kevin C Stein, Judith Frydman
Apr 24, 2019·ELife·Qiushuang WuAriel Alejandro Bazzini
Sep 19, 2019·RNA·Ashrut NarulaOlivia S Rissland
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Feb 11, 2021·Bio Systems·Julie D ThompsonChristian J Michel
Apr 29, 2021·Proceedings of the National Academy of Sciences of the United States of America·Sameer AryalEric Klann
Apr 30, 2021·Scientific Reports·Simone GianneriniAlberto Danielli
May 6, 2021·Wiley Interdisciplinary Reviews. RNA·Christelle MorrisEmiliano P Ricci

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