In this study, we have examined the influence of initiation factors on translation initiation of leaderless mRNAs whose 5'-terminal residues are the A of the AUG initiating codon. A 1:1 ratio of initiation factors to ribosomes abolished ternary complex formation at the authentic start codon of different leaderless mRNAs. Supporting this observation, in vitro translation assays using limiting ribosome concentrations with competing leaderless lambda cl and Escherichia coli ompA mRNAs, the latter containing a canonical ribosome binding site, revealed reduced cl synthesis relative to OmpA in the presence of added initiation factors. Using in vitro toeprinting and in vitro translation assays, we show that this effect can be attributed to IF3. Moreover, in vivo studies revealed that the translational efficiency of a leaderless reporter gene is decreased with increased IF3 levels. These studies are corroborated by the observed increased translational efficiency of a leaderless reporter construct in an infC mutant strain unable to discriminate against non-standard start codons. These results suggest that, in the absence of a leader or a Shine-Dalgarno sequence, the function(s) of IF3 limits stable 30S ternary complex formation.
Nucleic acid binding properties of Escherichia coli ribosomal protein S1. I. Structure and interactions of binding site I
In vivo translational start site selection on leaderless mRNA transcribed from the Streptomyces fradiae aph gene
Lack of a 5' non-coding region in Tn1721 encoded tetR mRNA is associated with a low efficiency of translation and a short half-life in Escherichia coli
Detection of Escherichia coli ribosome binding at translation initiation sites in the absence of tRNA
The anti-Shine-Dalgarno region in Escherichia coli 16S ribosomal RNA is not essential for the correct selection of translational starts
Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZ alpha and pUC18 or pUC19 multiple cloning sites
Cross-linking of initiation factor IF3 to Escherichia coli 30S ribosomal subunit by trans-diamminedichloroplatinum(II): characterization of two cross-linking sites in 16S rRNA; a possible way of functioning for IF3
Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis
AUU-to-AUG mutation in the initiator codon of the translation initiation factor IF3 abolishes translational autocontrol of its own gene (infC) in vivo
The unusual translational initiation codon AUU limits the expression of the infC (initiation factor IF3) gene of Escherichia coli
Determination of the RNA secondary structure that regulates lysis gene expression in bacteriophage MS2
The missing link in phage lysis of gram-positive bacteria: gene 14 of Bacillus subtilis phage phi 29 encodes the functional homolog of lambda S protein
Translation of vph mRNA in Streptomyces lividans and Escherichia coli after removal of the 5' untranslated leader
An AUG initiation codon, not codon-anticodon complementarity, is required for the translation of unleadered mRNA in Escherichia coli
Requirements for ribosomal protein S1 for translation initiation of mRNAs with and without a 5' leader sequence
Differential inhibition of 30S and 70S translation initiation complexes on leaderless mRNA by kasugamycin
Effects of ribosomal proteins S1, S2 and the DeaD/CsdA DEAD-box helicase on translation of leaderless and canonical mRNAs in Escherichia coli
Preferential selection of the 5'-terminal start codon on leaderless mRNAs by mammalian mitochondrial ribosomes.
Selective stimulation of translation of leaderless mRNA by initiation factor 2: evolutionary implications for translation
Role of the N- and C-terminal extensions on the activity of mammalian mitochondrial translational initiation factor 3
The roles of individual eukaryotic translation initiation factors in ribosomal scanning and initiation codon selection
Position of eukaryotic initiation factor eIF1 on the 40S ribosomal subunit determined by directed hydroxyl radical probing
High-resolution structures of ribosomal subunits: initiation, inhibition, and conformational variability
Characterization of the Streptomyces sp. strain C5 snp locus and development of snp-derived expression vectors
The initiation codon affects ribosome binding and translational efficiency in Escherichia coli of cI mRNA with or without the 5' untranslated leader
Isolation and characterization of ribosomes and translation initiation factors from the gram-positive soil bacterium Streptomyces lividans
Functional elements in initiation factors 1, 1A, and 2β discriminate against poor AUG context and non-AUG start codons
A leaderless mRNA can bind to mammalian 80S ribosomes and direct polypeptide synthesis in the absence of translation initiation factors
A 5'-terminal phosphate is required for stable ternary complex formation and translation of leaderless mRNA in Escherichia coli
Autoregulatory systems controlling translation factor expression: thermostat-like control of translational accuracy
70S-scanning initiation is a novel and frequent initiation mode of ribosomal translation in bacteria
A single mutation in the IF3 N-terminal domain perturbs the fidelity of translation initiation at three levels
Identification and role of functionally important motifs in the 970 loop of Escherichia coli 16S ribosomal RNA
Temporal regulation of viral transcription during development of Thermus thermophilus bacteriophage phiYS40
The role of interactions between phage and bacterial proteins within the infected cell: a diverse and puzzling interactome
Non-canonical RNA arrangement in T4-even phages: accommodated ribosome binding site at the gene 26-25 intercistronic junction
Requirements for translation re-initiation in Escherichia coli: roles of initiator tRNA and initiation factors IF2 and IF3
Multiple ways to regulate translation initiation in bacteria: Mechanisms, regulatory circuits, dynamics
The search and its outcome: high-resolution structures of ribosomal particles from mesophilic, thermophilic, and halophilic bacteria at various functional states
Leaderless Transcripts and Small Proteins Are Common Features of the Mycobacterial Translational Landscape
Non-canonical Escherichia coli transcripts lacking a Shine-Dalgarno motif have very different translational efficiencies and do not form a coherent group
Identification of mammalian mitochondrial translational initiation factor 3 and examination of its role in initiation complex formation with natural mRNAs.
Modulation of ribosomal recruitment to 5'-terminal start codons by translation initiation factors IF2 and IF3
Evidence for the translation initiation of leaderless mRNAs by the intact 70 S ribosome without its dissociation into subunits in eubacteria.
Biochemical and structural insights into RNA binding by Ssh10b, a member of the highly conserved Sac10b protein family in Archaea.
The Impact of Leadered and Leaderless Gene Structures on Translation Efficiency, Transcript Stability, and Predicted Transcription Rates in Mycobacterium smegmatis
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