Translation initiation factor AteIF(iso)4E is involved in selective mRNA translation in Arabidopsis thaliana seedlings.

PloS One
Ana Valeria Martínez-SilvaT D Dinkova

Abstract

One of the most regulated steps of translation initiation is the recruitment of mRNA by the translation machinery. In eukaryotes, this step is mediated by the 5'end cap-binding factor eIF4E bound to the bridge protein eIF4G and forming the eIF4F complex. In plants, different isoforms of eIF4E and eIF4G form the antigenically distinct eIF4F and eIF(iso)4F complexes proposed to mediate selective translation. Using a microarray analysis of polyribosome- and non-polyribosome-purified mRNAs from 15 day-old Arabidopsis thaliana wild type [WT] and eIF(iso)4E knockout mutant [(iso)4E-1] seedlings we found 79 transcripts shifted from polyribosomes toward non-polyribosomes, and 47 mRNAs with the opposite behavior in the knockout mutant. The translationally decreased mRNAs were overrepresented in root-preferentially expressed genes and proteins from the endomembrane system, including several transporters such as the phosphate transporter PHOSPHATE1 (PHO1), Sucrose transporter 3 (SUC3), ABC transporter-like with ATPase activity (MRP11) and five electron transporters, as well as signal transduction-, protein modification- and transcription-related proteins. Under normal growth conditions, eIF(iso)4E expression under the constitutive promote...Continue Reading

References

Aug 29, 1997·The Journal of Biological Chemistry·A Haghighat, N Sonenberg
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Apr 1, 2000·The Journal of Biological Chemistry·B D KeiperR E Rhoads
Oct 24, 2000·The Biochemical Journal·T D DinkovaE Sánchez de Jiménez
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·Anne DupratChristophe Robaglia
May 20, 2003·Current Opinion in Plant Biology·José López-BucioLuis Herrera-Estrella
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Nov 1, 2003·Science·Kayoko YamadaJoseph R Ecker
Feb 18, 2004·EMBO Reports·Marina PtushkinaJohn E G McCarthy
May 18, 2004·The Plant Journal : for Cell and Molecular Biology·Riki KawaguchiJulia Bailey-Serres
Jul 24, 2004·Biochemical Society Transactions·K S Browning
Apr 5, 2005·Mechanisms of Development·Greco HernándezRolando Rivera-Pomar
Jun 1, 2005·Mechanisms of Development·Greco Hernández, Paula Vazquez-Pianzola
Sep 30, 2005·BMC Evolutionary Biology·Bhavesh JoshiRosemary Jagus
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Keith W EarleyCraig S Pikaard
Feb 6, 2007·Nature·Popi SyntichakiNektarios Tavernarakis
Dec 7, 2007·Annals of the New York Academy of Sciences·Popi SyntichakiNektarios Tavernarakis
Dec 18, 2007·Molecular Cell·Nahum Sonenberg, Alan G Hinnebusch
Feb 25, 2009·The Journal of Biological Chemistry·Robert E Rhoads
May 6, 2009·The Journal of Biological Chemistry·Nicholas M KayeEckhard Jankowsky
May 21, 2009·The Plant Journal : for Cell and Molecular Biology·Maxwell S BushJohn H Doonan
Jul 15, 2009·Cell Cycle·Peter M Fischer
Jan 1, 2008·Advances in Bioinformatics·Tomas HruzPhilip Zimmermann
Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Richard J JacksonTatyana V Pestova
Feb 4, 2011·The Plant Journal : for Cell and Molecular Biology·Hatem RouachedYves Poirier
Feb 12, 2011·The Plant Journal : for Cell and Molecular Biology·Aleksandra StefanovicYves Poirier

❮ Previous
Next ❯

Citations

Aug 3, 2013·The Arabidopsis Book·Bijoyita Roy, Albrecht G von Arnim
Dec 21, 2012·Molecular Plant-microbe Interactions : MPMI·Carlos A Contreras-ParedesTzvetanka D Dinkova
Dec 27, 2015·Trends in Plant Science·Marten MooreKarl-Josef Dietz
Dec 3, 2014·Biochemical and Biophysical Research Communications·Anna KropiwnickaJoanna Zuberek
Aug 11, 2015·Frontiers in Plant Science·Elva C Chávez-HernándezTzvetanka D Dinkova
Nov 12, 2016·Frontiers in Microbiology·Masayoshi HashimotoShigetou Namba
Mar 5, 2017·Trends in Plant Science·Anna BastetJean-Luc Gallois
Mar 1, 2017·The Plant Journal : for Cell and Molecular Biology·Catharina MerchanteJose M Alonso
Apr 7, 2018·Wiley Interdisciplinary Reviews. RNA·Nikolay E Shirokikh, Thomas Preiss
Jan 17, 2017·Translation·Elizabeth LevinsKaren S Browning
May 6, 2020·Wiley Interdisciplinary Reviews. RNA·Ricardo A Urquidi CamachoAlbrecht G von Arnim
Jun 3, 2021·International Journal of Molecular Sciences·Eduardo Luján-SotoTzvetanka D Dinkova
May 17, 2021·Neurochemistry International·Aarti Sharma, Sidharth Mehan

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
reverse transcription PCR
PCR

Software Mentioned

GenArise
ScanArray 4000
ArrayPro Analyzer
MacVector
ImageJ
AtGenexpress Visualization

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.