PMID: 1204644Dec 1, 1975Paper

Translation of honeybee promelittin messenger RNA. Formation of a larger product in a mammalian cell-free system

European Journal of Biochemistry
G SuchanekG Kreil

Abstract

Venom glands of honeybees synthesize the peptide melittin via the precursor promelittin. Total RNA preparations from venom glands served as template in a cell-free system prepared from mammalian cells. The heterologous system translated the insect mRNA with approximately the same efficiency as hemoglobin mRNA. A polypeptide was synthesized which, as shown by acrylamide gel electrophoresis in the presence of detergent, has a higher molecular weight than promelittin. Analysis of peptic fragments as well as Edman degradation have demonstrated that sequences characteristic of venom gland promelittin are present in this product formed in vitro. Furthermore, a bacterial protease which specifically splits after acidic residues liberates from the cell-free product a fragment which closely resembles melittin. Evidence is presented that most of the extra amino acids are located at the amino terminus of the product formed in vitro. The larger polypeptide detected in vitro may represent a precursor of promelittin.

References

Sep 27, 1972·Nature: New Biology·C MilsteinM B Mathews
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J Houmard, G R Drapeau
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B MachP Vassalli
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·I Schechter
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·B KemperA Rich
Jan 1, 1972·Methods in Enzymology·K WeberM Osborn
Jun 11, 1971·European Journal of Biochemistry·G Kreil, H Bachmayer

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Citations

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S J ChanD F Steiner
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R L Jilka, S Pestka
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G Suchanek, G Kreil
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H Inouye, J Beckwith
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G SuchanekM A Hermodson
Jan 1, 1980·Annals of the New York Academy of Sciences·G KreilU Vilas
Mar 1, 1982·CRC Critical Reviews in Biochemistry·A W Strauss, I Boime
Nov 28, 1978·Biochemistry·D J McKean, R A Maurer
Jul 25, 1976·Journal of Molecular Biology·I Kindås-MüggeH Diggelmann
Aug 9, 1977·Biochimica Et Biophysica Acta·G C Shore, J R Tata
Aug 8, 1977·Biochemical and Biophysical Research Communications·J SekizawaM Inouye

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