PMID: 7543470Aug 1, 1995Paper

Translation of trpG in Bacillus subtilis is regulated by the trp RNA-binding attenuation protein (TRAP)

Journal of Bacteriology
M YangP Gollnick

Abstract

trpG of Bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and L-tryptophan. Expression of trpG is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. Examination of both transcriptional and translational gene fusions to lacZ involving trpG and direct measurements of trpG mRNA levels and TrpG polypeptide accumulation demonstrated that translation of trpG is regulated by tryptophan whereas transcription is not. these studies also show that this regulation is mediated by the trp RNA-binding attenuation protein. Deletion and point mutations indicated that regulation is dependent on a series of G/UAG trinucleotide repeats surrounding the putative ribosome-binding site for trpG. Our results are consistent with a model in which the tryptophan-activated trp RNA-binding attenuation protein and ribosomes compete for binding to trpG mRNA.

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Citations

Nov 16, 2005·Annual Review of Genetics·Paul GollnickCharles Yanofsky
Aug 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Joseph R SachlebenMark P Foster
Mar 19, 2015·Frontiers in Microbiology·Elke Van AsscheHans P Steenackers
Nov 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mikhail B ShevtsovAlfred A Antson
May 19, 2019·Annual Review of Microbiology·Paul BabitzkeTony Romeo
Mar 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·J P SarseroC Yanofsky
Oct 6, 2000·Annual Review of Microbiology·C L Squires, D Zaporojets
Oct 9, 1998·The Journal of Biological Chemistry·S XirasagarP A Gottlieb
Jan 24, 2002·The Journal of Biological Chemistry·Pan T X LiPaul Gollnick
Nov 5, 2005·Applied and Environmental Microbiology·T ZhuM M Domach
Jul 21, 2004·Journal of Bacteriology·Barbara C McCabe, Paul Gollnick
Jan 1, 1996·Biochimie·C YanofskyJ P Sarsero
Dec 14, 2004·Journal of Molecular Biology·Maria V BarbolinaPaul Gollnick
May 16, 2006·Structure·Jonathan G HeddleJeremy R H Tame
Mar 13, 2008·Journal of Molecular Biology·Yanling Chen, Paul Gollnick
Sep 26, 1997·Genes & Development·H R WilsonD L Court

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