Translation termination factors function outside of translation: yeast eRF1 interacts with myosin light chain, Mlc1p, to effect cytokinesis

Molecular Microbiology
Igor A ValouevM D Ter-Avanesyan

Abstract

The translation termination factor eRF1 recognizes stop codons at the A site of the ribosome and induces peptidyl-tRNA hydrolysis at the peptidyl transferase centre. Recent data show that, besides translation, yeast eRF1 is also involved in cell cycle regulation. To clarify the mechanisms of non-translational functions of eRF1, we performed a genetic screen for its novel partner proteins. This screen revealed the gene for myosin light chain, Mlc1p, acting as a dosage suppressor of a temperature-sensitive mutation in the SUP45 gene encoding eRF1. eRF1 and Mlc1p are able to interact with each other and, similarly to depletion of Mlc1p, mutations in the SUP45 gene may affect cytokinesis. Immunofluorescent staining performed to determine localization of Mlc1p has shown that the sup45 mutation, which arrests cytokinesis, redistributed Mlc1p, causing its disappearance from the bud tip and the bud neck. The data obtained demonstrate that yeast eRF1 has an important non-translational function effecting cytokinesis via interaction with Mlc1p.

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Citations

May 7, 2010·Nucleic Acids Research·Gloria H MerrittTobias von der Haar
Jan 19, 2012·PloS One·Natalia V Kochneva-PervukhovaMichael D Ter-Avanesyan
Jun 2, 2005·Reviews of Physiology, Biochemistry and Pharmacology·S RospertY Dubaquié
Dec 18, 2015·Scientific Reports·Genrikh V SerpionovMichael D Ter-Avanesyan
Dec 26, 2006·Trends in Microbiology·Tobias von der Haar, Mick F Tuite
Jul 15, 2015·Bioarchitecture·Sarah M Heissler, James R Sellers
Aug 18, 2009·Molecular Microbiology·Namitha VishveshwaraSusan W Liebman
May 31, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroyuki KodamaYoshikazu Nakamura
Aug 19, 2006·Cell Motility and the Cytoskeleton·Josef D FrankeDaniel P Kiehart
May 4, 2017·The FEBS Journal·Valery N UrakovMichael D Ter-Avanesyan
Jan 12, 2019·Prion·Vitaly V Kushnirov
Feb 3, 2005·Yeast
Nov 23, 2018·International Journal of Molecular Sciences·Valery N UrakovMichael D Ter-Avanesyan

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