Translational activation by the noncoding RNA DsrA involves alternative RNase III processing in the rpoS 5'-leader

RNA
Armin ReschVladimir R Kaberdin

Abstract

The intricate regulation of the Escherichia coli rpoS gene, which encodes the stationary phase sigma-factor sigmaS, includes translational activation by the noncoding RNA DsrA. We observed that the stability of rpoS mRNA, and concomitantly the concentration of sigmaS, were significantly higher in an RNase III-deficient mutant. As no decay intermediates corresponding to the in vitro mapped RNase III cleavage site in the rpoS leader could be detected in vivo, the initial RNase III cleavage appears to be decisive for the observed rapid inactivation of rpoS mRNA. In contrast, we show that base-pairing of DsrA with the rpoS leader creates an alternative RNase III cleavage site within the rpoS/DsrA duplex. This study provides new insights into regulation by small regulatory RNAs in that the molecular function of DsrA not only facilitates ribosome loading on rpoS mRNA, but additionally involves an alternative processing of the target.

References

Apr 1, 1986·Molecular & General Genetics : MGG·S Lin-Chao, H Bremer
Dec 15, 2000·Molecular Microbiology·R A Lease, M Belfort
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
May 7, 2003·Current Opinion in Microbiology·Eric MasséSusan Gottesman
Sep 17, 2003·Genes & Development·Eric MasséSusan Gottesman
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Neema AgrawalSunil K Mukherjee
Jan 24, 2004·The EMBO Journal·Thomas A Geissmann, Danièle Touati
Mar 11, 2004·Molecular Microbiology·Poul Valentin-HansenChristina Udesen
Nov 25, 2004·Journal of Molecular Biology·Richard A Lease, Sarah A Woodson
Dec 29, 2004·Current Biology : CB·Jörg VogelShoshy Altuvia
Jan 5, 2005·Biochemical and Biophysical Research Communications·Vladimir R Kaberdin, Thierry Bizebard
Apr 6, 2005·Nature Structural & Molecular Biology·Carin K Vanderpool, Susan Gottesman
May 26, 2005·Trends in Genetics : TIG·Susan Gottesman
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Teppei MoritaHiroji Aiba
Jul 4, 2006·Journal of Bacteriology·Amy M JonesThomas Elliott
Sep 23, 2006·FEMS Microbiology Reviews·Vladimir R Kaberdin, Udo Bläsi

❮ Previous
Next ❯

Citations

Dec 21, 2012·Future Microbiology·Rebekka Steinmann, Petra Dersch
Jun 7, 2011·Annual Review of Microbiology·Aurelia BattestiSusan Gottesman
Nov 26, 2009·International Journal of Microbiology·Kelsi L Anderson, Paul M Dunman
Jan 15, 2009·Genes & Development·Kwang-sun KimStanley N Cohen
Dec 15, 2010·Nucleic Acids Research·Mark B SteadSidney R Kushner
Jun 22, 2012·Nucleic Acids Research·Euripedes de Almeida RibeiroKristina Djinović-Carugo
Aug 19, 2010·Molecular Genetics and Genomics : MGG·Ramakanth MadhugiriGabriele Klug
Mar 19, 2008·Applied Microbiology and Biotechnology·Zhen KangQingsheng Qi
Oct 27, 2012·FEBS Letters·Mercedes de la FuenteJosé Luis Martínez-Guitarte
Nov 3, 2009·Current Opinion in Microbiology·Kathrin S Fröhlich, Jörg Vogel
Dec 15, 2010·Molecular Microbiology·Dimitri Podkaminski, Jörg Vogel
Jan 8, 2013·Biochimica Et Biophysica Acta·Vladimir R Kaberdin, Udo Bläsi
May 3, 2015·FEMS Microbiology Reviews·Kai Papenfort, Carin K Vanderpool
Apr 8, 2014·Current Opinion in Microbiology·Margarida SaramagoCecília M Arraiano
Sep 16, 2010·FEMS Microbiology Letters·Barbara O GvakhariaDaniel J Arp
Apr 27, 2013·FEMS Microbiology Letters·Tatiana RochatFrancis Repoila
Feb 1, 2011·Journal of Bacteriology·Asaf Peer, Hanah Margalit
Apr 11, 2018·Journal of Bacteriology·Melissa L AnackerD Scott Samuels
Sep 4, 2009·Acta Biochimica Et Biophysica Sinica·Yinghua Zhang, Guofan Hong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.