Translational activation of maternal mRNA encoding the heat-shock protein hsp90 during sea urchin embryogenesis

Developmental Biology
P A Bédard, B P Brandhorst

Abstract

Changes in protein synthesis induced by heat shock of Strongylocentrotus purpuratus gastrulae were analyzed bt two-dimensional electrophoresis. Hyperthermia induces the synthesis of polypeptides having molecular masses of 90, 70, 50, 40, and 38 kDa. One of these, hsp90, appears as a pair of polypeptides which comigrates with proteins synthesized at normal temperature in eggs and embryos; these comigrating spots produce indistinguishable patterns upon electrophoretic analysis of partial V8 protease digests, indicating that hsp90 is synthesized throughout embryogenesis. The relative rate of incorporation of methionine into hsp90 is low in eggs and zygotes, but increases abruptly in morulae, constituting a rare and striking change in protein synthesis during early development. Cell-free translation analyses indicate that most of the mRNA encoding hsp90 resides in the pool of free ribonucleoprotein particles in eggs and early embryos, but shifts to polysomes by the 64-cell stage while remaining constant in mass. Thus the increase in synthesis of hsp90 appears to be via the selective activation of translation of a stored maternal mRNA. The shift of hsp90 mRNA to polysomes is accompanied by polyadenylation. Heat shock of eggs or zygo...Continue Reading

References

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Citations

Feb 1, 1991·Molecular Reproduction and Development·Z Y GongB P Brandhorst
Jul 16, 1990·Gene·D Munroe, A Jacobson
Nov 1, 1994·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·B M Sanders, L S Martin
Aug 4, 2012·Molecular Ecology·Daniel E RuncieGregory A Wray
Oct 25, 2016·Molecular Reproduction and Development·Vincent PicardRobert Bellé
Aug 31, 1999·Development, Growth & Differentiation·G GiudiceM C Roccheri
Jul 1, 1990·Molecular Reproduction and Development·M B Dworkin, E Dworkin-Rastl
Jan 1, 1993·Critical Reviews in Toxicology·B M Sanders
Jun 1, 1989·Molecular and Cellular Biology·E HickeyL A Weber

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