PMID: 6165016Mar 1, 1981Paper

Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences

Proceedings of the National Academy of Sciences of the United States of America
H SoreqP B Sehgal

Abstract

Polyadenylylated mRNA was purified from poly(I).poly(C)- and cycloheximide-superinduced human fibroblast (FS-4) cultures. The mRNA was subjected to electrophoresis through an agarose/CH3HgOH gel, and human fibroblast beta 1 and beta 2 interferon mRNAs were isolated. Each mRNA preparation was phosphorolyzed at 0 degrees C for 20 min by using a molar excess of polynucleotide phosphorylase to produce RNAs lacking poly(A) and then incubated at 37 degrees C for varying lengths of time to allow the phosphorylase to further digest the deadenylylated RNA from the 3' end in a processive and synchronous manner. Removal of the poly(A) (less than or equal to 100 residues) and approximately 100 adjacent residues from human fibroblast beta 1 interferon mRNA (native length, 900 residues, including a 3'-noncoding region of 203 residues) did not alter the translational activity or the functional stability of this mRNA in Xenopus oocytes, whereas deletion of the poly(A) and approximately 200 adjacent residues decreased its translational efficiency. On the other hand, removal of the poly(A) (approximately 200 residues) and approximately 200 adjacent residues from human fibroblast beta 2 interferon mRNA (native length, 1300 residues) did not alter...Continue Reading

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Citations

Sep 1, 1992·Brain Research. Brain Research Reviews·B J Snider, M Morrison-Bogorad
Sep 1, 1989·Trends in Biochemical Sciences·P Bernstein, J Ross
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H SoreqI Silman
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J ContentW Fiers
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A DarveauN Sonenberg
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Feb 1, 1982·Journal of Neurochemistry·D Eliyahu, H Soreq
Jan 1, 1985·CRC Critical Reviews in Biochemistry·H Soreq
Sep 30, 1982·Biochimica Et Biophysica Acta·P B Sehgal

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