Translational Advances of Hydrofection by Hydrodynamic Injection

Genes
Luis SendraSalvador F Aliño

Abstract

Hydrodynamic gene delivery has proven to be a safe and efficient procedure for gene transfer, able to mediate, in murine model, therapeutic levels of proteins encoded by the transfected gene. In different disease models and targeting distinct organs, it has been demonstrated to revert the pathologic symptoms and signs. The therapeutic potential of hydrofection led different groups to work on the clinical translation of the procedure. In order to prevent the hemodynamic side effects derived from the rapid injection of a large volume, the conditions had to be moderated to make them compatible with its use in mid-size animal models such as rat, hamster and rabbit and large animals as dog, pig and primates. Despite the different approaches performed to adapt the conditions of gene delivery, the results obtained in any of these mid-size and large animals have been poorer than those obtained in murine model. Among these different strategies to reduce the volume employed, the most effective one has been to exclude the vasculature of the target organ and inject the solution directly. This procedure has permitted, by catheterization and surgical procedures in large animals, achieving protein expression levels in tissue close to those ac...Continue Reading

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Citations

Sep 27, 2020·Genes·Ilayda AtesRenee N Cottle
Mar 5, 2019·Biological & Pharmaceutical Bulletin·Yağız Anıl ÇiçekVincent M Rotello
Dec 10, 2020·Nature Communications·Jared Carlson-StevermerKrishanu Saha
May 23, 2020·Clinics in Laboratory Medicine·Elan Hahn, Matthew Hiemenz
Jun 24, 2021·JHEP Reports : Innovation in Hepatology·Sheila MaestroGloria Gonzalez-Aseguinolaza
Aug 8, 2021·Cells·Niloofar Khoshdel-RadMassoud Vosough
Sep 13, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gregory A Newby, David R Liu
Nov 17, 2021·Immunotherapy·Constanza Savid-FronteraMaria C Rodriguez-Galan

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Methods Mentioned

BETA
transfection
electron microscopy
transmission electron microscopy
PCR
ELISA
protein folding
surgical resection
hydrofection

Software Mentioned

Organox

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