Translational arrest by a prokaryotic signal recognition particle is mediated by RNA interactions

Nature Structural & Molecular Biology
Bertrand BeckertRoland Beckmann

Abstract

The signal recognition particle (SRP) recognizes signal sequences of nascent polypeptides and targets ribosome-nascent chain complexes to membrane translocation sites. In eukaryotes, translating ribosomes are slowed down by the Alu domain of SRP to allow efficient targeting. In prokaryotes, however, little is known about the structure and function of Alu domain-containing SRPs. Here, we report a complete molecular model of SRP from the Gram-positive bacterium Bacillus subtilis, based on cryo-EM. The SRP comprises two subunits, 6S RNA and SRP54 or Ffh, and it facilitates elongation slowdown similarly to its eukaryotic counterpart. However, protein contacts with the small ribosomal subunit observed for the mammalian Alu domain are substituted in bacteria by RNA-RNA interactions of 6S RNA with the α-sarcin-ricin loop and helices H43 and H44 of 23S rRNA. Our findings provide a structural basis for cotranslational targeting and RNA-driven elongation arrest in prokaryotes.

References

Sep 25, 1984·Journal of Molecular Biology·S Brown, M J Fournier
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·H TjalsmaJ M van Dijl
Oct 31, 2002·Molecular Microbiology·Keith Stephenson, James A Hoch
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Harold TjalsmaJan Maarten van Dijl
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Jul 28, 2006·Journal of Structural Biology·James Z Chen, Nikolaus Grigorieff
Jul 11, 2008·Journal of Structural Biology·J J FernándezJ L Carrascosa
Jan 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xin ZhangShu-ou Shan
Oct 20, 2009·RNA Biology·Magnus Alm RosenbladChristian Zwieb
Apr 7, 2010·Nature·Claudia Y JandaKiyoshi Nagai
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Jul 31, 2012·Nature Methods·Sjors H W Scheres, Shaoxia Chen
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 13, 2012·Nature Structural & Molecular Biology·Wolf HoltkampWolfgang Wintermeyer
Feb 19, 2013·Annual Review of Biochemistry·David AkopianShu-ou Shan
Mar 14, 2013·Biochimica Et Biophysica Acta·Yvonne NyathiMartin R Pool
May 3, 2013·Nature·Andreas M AngerRoland Beckmann
Nov 12, 2013·Molecular Cell·Felix Voigts-HoffmannNenad Ban
Apr 5, 2014·Science·Jan Timo GrotwinkelIrmgard Sinning

❮ Previous
Next ❯

Citations

Dec 16, 2016·Cell Reports·Alexej KedrovRoland Beckmann
Dec 3, 2016·Nucleic Acids Research·Matthias M M BeckerIrmgard Sinning
Sep 30, 2016·Microbiology and Molecular Biology Reviews : MMBR·Daniel R ReußJörg Stülke
Aug 4, 2016·Nature·Justin W ChartronJudith Frydman
May 28, 2019·The Protein Journal·Dries SmetsAnastassios Economou
Sep 21, 2019·The Journal of Cell Biology·Yu-Hsien Hwang FuShu-Ou Shan
Aug 14, 2019·Biological Chemistry·Klemens WildIrmgard Sinning
Dec 3, 2016·Current Microbiology·Sayandeep GuptaAbhrajyoti Ghosh
May 24, 2018·FEMS Microbiology Letters·Ruth SteinbergHans-Georg Koch
Feb 13, 2020·Plants·Fabian RiesFelix Willmund
Dec 2, 2020·Molecular Cell·Caillan Crowe-McAuliffeVasili Hauryliuk
May 1, 2021·Frontiers in Molecular Biosciences·Chen JiangDamon Huber

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.