PMID: 9443976Mar 14, 1998Paper

Translational attenuation of the Bacillus subtilis spo0B cistron by an RNA structure encompassing the initiation region

Nucleic Acids Research
M AsayamaY Kobayashi

Abstract

The spo0B gene, which exists as an operon with the obg gene, is required to initiate sporulation (stage 0) of Bacillus subtilis . This gene encodes a phosphotransferase in the multicomponent phosphorelay system. We here report the novel finding that a spo0B 5'-terminal SLR (stem-loop structure sequestering ribosome binding sequence; ACUCCUAA-X16-UUG GGAG U, Delta G = -8.71 kcal/mol) attenuated spo0B translation. The spo0B gene was efficiently transcribed but Spo0B protein was not overproduced in Escherichia coli when spo0B was induced using expression vectors carrying the SLR- spo0B region under control of the tac promoter. Deletion of the SLR from the vectors resulted in overexpression of spo0B . Therefore, to characterize expression of spo0B with a SLR in B.subtilis we constructed transcriptional and translational lacZ fusions combined with the spo0B 5'-terminal region with a deleted or mutagenized SLR. These constructs were subsequently introduced into B.subtilis as multiple and single copies, then beta-galactosidase activities were measured. The possible SLR also functioned as a negative cis element in B.subtilis. Furthermore, B.subtilis strain 1S16 (spo0B136) lysogenized straight phiCD0B-S and -W, harboring spo0B with muta...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1992·Genes & Development·S A EmoryJ G Belasco
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Zhang, M P Deutscher
May 25, 1991·Proceedings of the National Academy of Sciences of the United States of America·S SatolaC P Moran
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M StrauchJ A Hoch
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C TuerkI Tinoco
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E S MasudaY Kobayashi
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H ShimotsuH Saito
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J BouvierJ Szulmajster
Mar 1, 1993·Molecular Microbiology·J F DiMari, D H Bechhofer

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