Translational autoregulation of BZW1 and BZW2 expression by modulating the stringency of start codon selection.

PloS One
Gary LoughranIvaylo P Ivanov

Abstract

The efficiency of start codon selection during ribosomal scanning in eukaryotic translation initiation is influenced by the context or flanking nucleotides surrounding the AUG codon. The levels of eukaryotic translation initiation factors 1 (eIF1) and 5 (eIF5) play critical roles in controlling the stringency of translation start site selection. The basic leucine zipper and W2 domain-containing proteins 1 and 2 (BZW1 and BZW2), also known as eIF5-mimic proteins, are paralogous human proteins containing C-terminal HEAT domains that resemble the HEAT domain of eIF5. We show that translation of mRNAs encoding BZW1 and BZW2 homologs in fungi, plants and metazoans is initiated by AUG codons in conserved unfavorable initiation contexts. This conservation is reminiscent of the conserved unfavorable initiation context that enables autoregulation of EIF1. We show that overexpression of BZW1 and BZW2 proteins enhances the stringency of start site selection, and that their poor initiation codons confer autoregulation on BZW1 and BZW2 mRNA translation. We also show that overexpression of these two proteins significantly diminishes the effect of overexpressing eIF5 on stringency of start codon selection, suggesting they antagonize this func...Continue Reading

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Citations

Sep 6, 2018·BMC Structural Biology·Jielin Yu, Assen Marintchev
May 6, 2020·Wiley Interdisciplinary Reviews. RNA·Ricardo A Urquidi CamachoAlbrecht G von Arnim
Sep 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Gary LoughranDmitry E Andreev
Jan 5, 2021·Bioscience Reports·Yabin LiRuijian Zhang

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Methods Mentioned

BETA
transfections
transfection
Assay

Software Mentioned

ClustalX
tBLASTn

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