PMID: 11333027May 3, 2001Paper

Translational control by delayed RNA folding: identification of the kinetic trap

RNA
D van MeertenJ van Duin

Abstract

The maturation or A-protein gene of single-stranded RNA phage MS2 is preceded by a 130-nt long untranslated leader. When MS2 RNA folding is at equilibrium, the gene is untranslatable because the leader adopts a well-defined cloverleaf structure in which the Shine-Dalgarno (SD) sequence of the maturation gene is taken up in long-distance base pairing with an upstream complementary sequence (UCS). Synthesis of the A-protein takes place transiently while the RNA is synthesized from the minus strand. This requires that formation of the inhibitory cloverleaf is slow. In vitro, the folding delay was on the order of minutes. Here, we present evidence that this postponed folding is caused by the formation of a metastable intermediate. This intermediate is a small local hairpin that contains the UCS in its loop, thereby preventing or slowing down its pairing with the SD sequence. Mutants in which the small hairpin could not be formed made no detectable amounts of A-protein and were barely viable. Apparently, here the cloverleaf formed quicker than ribosomes could bind. On the other hand, mutants in which the small intermediary hairpin was stabilized produced more A-protein than wild type and were viable. One hardly growing mutant that c...Continue Reading

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Citations

Nov 30, 2002·Biochimie·Jord H A Nagel, Cornelis W A Pleij
Dec 21, 2006·Nucleic Acids Research·A XayaphoummineH Isambert
Jul 21, 2006·Nucleic Acids Research·J H A NagelC W A Pleij
Apr 30, 2013·Nucleic Acids Research·Jing Yun A ZhuIrmtraud M Meyer
Jun 28, 2002·Nucleic Acids Research·J H A NagelC W A Pleij
May 28, 2004·Nucleic Acids Research·Michael PaulusManfred Watzele
Apr 24, 2009·Annual Review of Microbiology·Paul BabitzkeTony Romeo
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alain LaederachMichael Brenowitz
Apr 29, 2008·Journal of Molecular Biology·Michael GeisCaroline Thurner
May 26, 2007·Virus Research·Rosa M PintóAlbert Bosch
Sep 3, 2005·Chembiochem : a European Journal of Chemical Biology·Hashim M Al-Hashimi
Jun 6, 2003·Molecular Microbiology·Paula Jean Schlax, David J Worhunsky
Oct 3, 2003·Chembiochem : a European Journal of Chemical Biology·Ronald Micura, Claudia Höbartner
Jan 15, 2005·Virus Research·Christof K Biebricher, Manfred Eigen
Mar 10, 2015·RNA Biology·Jing Yun A Zhu, Irmtraud M Meyer
Aug 27, 2004·Journal of Molecular Biology·Jeremy Flinders, Thorsten Dieckmann
Apr 19, 2002·The Journal of General Virology·Dico van MeertenJan van Duin
Jan 29, 2019·Briefings in Functional Genomics·Tsukasa Fukunaga, Michiaki Hamada
May 12, 2019·Nucleic Acids Research·Shungo KoboriYohei Yokobayashi
Oct 20, 2020·Physical Biology·Wasiur R KhudaBukhshGrzegorz A Rempała

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