Translational pancreatic cancer research: A comparative study on patient-derived xenograft models

World Journal of Gastroenterology : WJG
Mercedes Rubio-Manzanares DoradoJavier Padillo Ruiz

Abstract

To assess the viability of orthotopic and heterotopic patient-derived pancreatic cancer xenografts implanted into nude mice. This study presents a prospective experimental analytical follow-up of the development of tumours in mice upon implantation of human pancreatic adenocarcinoma samples. Specimens were obtained surgically from patients with a pathological diagnosis of pancreatic adenocarcinoma. Tumour samples from pancreatic cancer patients were transplanted into nude mice in three different locations (intraperitoneal, subcutaneous and pancreatic). Histological analysis (haematoxylin-eosin and Masson's trichrome staining) and immunohistochemical assessment of apoptosis (TUNEL), proliferation (Ki-67), angiogenesis (CD31) and fibrogenesis (α-SMA) were performed. When a tumour xenograft reached the target size, it was re-implanted in a new nude mouse. Three sequential tumour xenograft generations were generated (F1, F2 and F3). The overall tumour engraftment rate was 61.1%. The subcutaneous model was most effective in terms of tissue growth (69.9%), followed by intraperitoneal (57.6%) and pancreatic (55%) models. Tumour development was faster in the subcutaneous model (17.7 ± 2.6 wk) compared with the pancreatic (23.1 ± 2.3 wk...Continue Reading

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Citations

Apr 17, 2020·Physiological Reviews·Elisabeth HessmannAlbrecht Neesse
Oct 7, 2021·Journal of Magnetic Resonance Imaging : JMRI·YeYu CaiEnHua Xiao

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Methods Mentioned

BETA
xenograft
xenografts
biopsy
pancreatectomy
nuclear magnetic resonance
SMA

Software Mentioned

Cell Sens Dimensions
Cut
off Finder
SPSS

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis