PMID: 8995616Feb 1, 1997Paper

Translational regulation of the human cytomegalovirus pp28 (UL99) late gene

Journal of Virology
J A KerryR M Stenberg

Abstract

The pp28 (UL99) gene of human cytomegalovirus is expressed as a true late gene, in that DNA synthesis is absolutely required for mRNA expression. Our previous studies demonstrated that pp28 promoter sequences from position -40 to +106 are sufficient for late gene expression in the context of the viral genome (C. P. Kohler, J. A. Kerry, M. Carter, V. P. Muzithras, T. R. Jones, and R. M. Stenberg, J. Virol. 68:6589-6597, 1994). To extend these studies, we have examined the sequences in the downstream leader region of the pp28 gene for their role in late gene expression. Deletion of sequences from position -6 to +46 (deltaSS) results in a threefold increase in gene expression in transient assays. In contrast, deletion of sequences from position +46 to +88 (deltaA) has little effect on gene expression. These results indicate that the sequences from position -6 to +46 may repress gene expression. To further analyze this region, site-directed mutagenesis was performed. Mutation of residues from either position +1 to +6 (SS1) or position +12 to +17 (SS2) duplicated the effect of the deltaSS deletion mutant, indicating that sequences from position +1 to +17 were important for the inhibitory effect. To assess the biological significance...Continue Reading

References

Jan 1, 1992·Journal of Virology·A M Colberg-PoleyD J Tenney
Dec 1, 1991·Journal of Virology·M R SchleissA P Geballe
Aug 15, 1991·Biochemical and Biophysical Research Communications·K KashiwagiK Igarashi
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May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M Kozak
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Jan 1, 1984·Journal of Virology·R M StenbergM F Stinski
May 1, 1994·Molecular Biology Reports·R J JacksonA Kaminski

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Citations

May 31, 2013·Journal of Virology·Shinya Omoto, Edward S Mocarski
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ann-Charlotte IversenChris A Benedict
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Robert F Kalejta
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Caleb McKinneyIan Mohr
Jan 9, 1999·Journal of Virology·N H ChauJ A Kerry

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